Analytical Data
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基因名
TRIM5
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简介
The TRIM5 protein is a capsid-specific restriction factor that prevents non-host adapted retroviral infection. TRIM5 acts as a pattern recognition receptor that activates innate immune signaling by triggering E3 ubiquitin ligase activity upon binding to viral capsids. TRIM5 restricts various retroviruses and regulates autophagy. TRIM5 Protein, Human (N-His) is the recombinant human-derived TRIM5 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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别名
Tripartite motif-containing protein 5; RING finger protein 88; TRIM5; RNF88
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q9C035-1
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表达区间
M1-Q248
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氨基酸序列
MASGILVNVKEEVTCPICLELLTQPLSLDCGHSFCQACLTANHKKSMLDKGESSCPVCRISYQPENIRPNRHVANIVEKLREVKLSPEGQKVDHCARHGEKLLLFCQEDGKVICWLCERSQEHRGHHTFLTEEVAREYQVKLQAALEMLRQKQQEAEELEADIREEKASWKTQIQYDKTNVLADFEQLRDILDWEESNELQNLEKEEEDILKSLTNSETEMVQQTQSLRELISDLEHRLQGSVMELLQ
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蛋白长度
Partial
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分子量
30 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TRIM5 is a member of the tripartite motif (TRIM) family of proteins, known for its role in the innate immune response against retroviruses. The interest in TRIM5 research has escalated particularly due to its unique ability to recognize and restrict various retroviruses, including HIV-1, which poses significant challenges to global health. TRIM5 functions as a restriction factor by binding to the capsid of the incoming viral particles, leading to their recognition and subsequent degradation by the cellular proteasome. Understanding the mechanisms of TRIM5's antiviral activity is critical for the development of novel therapeutic strategies against retroviral infections. Additionally, variations in TRIM5 alleles among different primate species provide insights into the evolutionary pressures exerted by retroviruses, showcasing a fascinating interplay between host defense mechanisms and viral evasion strategies. Recent studies have also explored the potential of engineering TRIM5 proteins to enhance their antiviral efficacy, aiming to create new avenues for gene therapy and vaccine development. Thus, ongoing research into TRIM5 not only enriches our understanding of host-pathogen interactions but also holds promise for innovative approaches to combat retroviral diseases.
