Analytical Data
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Gene name
TDT
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简介
TDT protein, a template-independent DNA polymerase, non-specifically adds deoxynucleoside 5'-triphosphates to the 3'-end of a DNA initiator. It crucially contributes to in vivo processes, particularly adding nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during B- and T-cell maturation. TDT Protein, Human (His) is the recombinant human-derived TDT protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltr; Terminal deoxynucleotidyltransferase; Terminal transferase; DNTT; TDT
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P04053
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Expression Region
M1-A509
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AA Sequence
MDPPRASHLSPRKKRPRQTGALMASSPQDIKFQDLVVFILEKKMGTTRRAFLMELARRKGFRVENELSDSVTHIVAENNSGSDVLEWLQAQKVQVSSQPELLDVSWLIECIRAGKPVEMTGKHQLVVRRDYSDSTNPGPPKTPPIAVQKISQYACQRRTTLNNCNQIFTDAFDILAENCEFRENEDSCVTFMRAASVLKSLPFTIISMKDTEGIPCLGSKVKGIIEEIIEDGESSEVKAVLNDERYQSFKLFTSVFGVGLKTSEKWFRMGFRTLSKVRSDKSLKFTRMQKAGFLYYEDLVSCVTRAEAEAVSVLVKEAVWAFLPDAFVTMTGGFRRGKKMGHDVDFLITSPGSTEDEEQLLQKVMNLWEKKGLLLYYDLVESTFEKLRLPSRKVDALDHFQKCFLIFKLPRQRVDSDQSSWQEGKTWKAIRVDLVLCPYERRAFALLGWTGSRQFERDLRRYATHERKMILDNHALYDKTKRIFLKAESEEEIFAHLGLDYIEPWERNA
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Protein Length
Full Length
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Molecular Weight
62.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of TDT (Terminal deoxynucleotidyl transferase) recombinant protein has garnered significant interest in the fields of molecular biology and biochemistry due to its crucial role in DNA manipulation and synthesis. TDT is a DNA polymerase that catalyzes the addition of nucleotides to the 3' ends of a DNA molecule, making it essential for various applications, including in vitro DNA amplification, gene therapy, and the construction of DNA libraries. Its ability to add nucleotides without a template allows for the generation of homopolymeric tails on DNA fragments, which can enhance cloning efficiency and facilitate the modification of DNA sequences. The advent of recombinant DNA technology has enabled the production of TDT in high yields, providing researchers with a more accessible and potent tool for genetic engineering. Moreover, understanding TDT's structure and function at the molecular level can lead to the development of novel applications in synthetic biology, diagnostics, and therapeutics. As research progresses, the insights gained from studying TDT recombinant proteins not only enhance our comprehension of DNA biology but also pave the way for innovative approaches in genome editing and personalized medicine.
