Analytical Data
-
基因名
Tissue alpha-L-Fucosidase
-
简介
Tissue alpha-L-Fucosidase Proteinas, an enzymatic entity, crucially hydrolyzes alpha-1,6-linked fucose residues on glycoproteins' carbohydrate moieties. Tissue alpha-L-Fucosidase Protein, Mouse (His-SUMO) is the recombinant mouse-derived Tissue alpha-L-Fucosidase protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
- Application
-
别名
Fuca1; FucaTissue alpha-L-fucosidase; EC 3.2.1.51; Alpha-L-fucosidase I; Alpha-L-fucoside fucohydrolase 1; Alpha-L-fucosidase 1
-
种属
Mouse
-
表达系统
E. coli
-
标签
N-His;N-SUMO
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q99LJ1
-
表达区间
L18-N452
-
氨基酸序列
LAPRRFTPDWQSLDSRPLPSWFDEAKFGVFVHWGVFSVPAWGSEWFWWHWQGDRMPAYQRFMTENYPPGFSYADFAPQFTARFFHPDQWAELFQAAGAKYVVLTTKHHEGFTNWPSPVSWNWNSKDVGPHRDLVGELGAAVRKRNIRYGLYHSLLEWFHPLYLLDKKNGFKTQHFVRAKTMPELYDLVNSYKPDLIWSDGEWECPDTYWNSTSFLAWLYNDSPVKDEVIVNDRWGQNCSCHHGGYYNCQDKYKPQSLPDHKWEMCTSMDRASWGYRKDMTMSTIAKENEIIEELVQTVSLGGNYLLNIGPTKDGLIVPIFQERLLAVGKWLQINGEAIYASKPWRVQSEKNKTVVWYTTKNATVYATFLYWPENGIVNLKSPKTTSATKITMLGLEGDLSWTQDPLEGVLISLPQLPPTVLPVEFAWTLKLTKVN
-
蛋白长度
Full Length of Mature Protein
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Tissue alpha-L-fucosidase is an important enzyme involved in glycoprotein degradation and is essential for various biological processes, including cellular communication and immune response. Deficiency in this enzyme is associated with genetic disorders, such as fucosidosis, which leads to the accumulation of fucosylated compounds and results in severe clinical manifestations. Research into the recombinant expression of tissue alpha-L-fucosidase is crucial for understanding its structure, function, and potential therapeutic applications. By producing this enzyme in a recombinant system, researchers aim to obtain sufficient quantities for enzymatic characterization, elucidate its catalytic mechanisms, and explore its role in disease pathology. Additionally, recombinant alpha-L-fucosidase could pave the way for developing enzyme replacement therapies for patients with fucosidosis and other related disorders. Advances in recombinant DNA technology have facilitated the expression of this enzyme in various host systems, enabling optimized production processes and functional studies. Thus, the exploration of recombinant tissue alpha-L-fucosidase not only contributes to the fundamental biochemistry of glycan metabolism but also holds promise for clinical advancements in treating disorders linked to fucose metabolism.
