Analytical Data
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基因名
RecO
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简介
RecO protein, a crucial participant in DNA repair and the RecF pathway, operates as a monomer, contributing to the intricate choreography of DNA repair mechanisms and facilitating recombination. Its monomeric form underscores its singular involvement, highlighting its significance in orchestrating cellular responses to DNA damage and recombination. RecO Protein, E.coli (His-SUMO) is the recombinant E. coli-derived RecO protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag.
- Application
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别名
recO; b2565; JW2549; DNA repair protein RecO; Recombination protein O
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种属
E.coli
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表达系统
E. coli
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标签
N-SUMO;N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0A7H3
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表达区间
M1-E242
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氨基酸序列
MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRSTLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEYETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDDTMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLGGKPLKSRELFRQFMPKRTVKTHYE
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蛋白长度
Full Length
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分子量
43.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RecO proteins are essential components of the DNA repair and recombination machinery in bacteria. They play a critical role in promoting homologous recombination, a process that is crucial for maintaining genomic integrity, especially under conditions of DNA damage. The study of RecO proteins has gained significant attention due to their involvement in repairing double-strand breaks and facilitating the exchange of genetic material, which is vital for bacterial evolution and adaptation. Understanding the mechanisms by which RecO proteins function can provide insights into bacterial survival strategies, particularly in hostile environments, and may reveal potential targets for novel antimicrobial therapies. Furthermore, the study of RecO and its interactions with other recombination proteins, such as RecA, highlights the complexity and efficiency of bacterial DNA repair systems. Insights gained from RecO research not only contribute to our fundamental understanding of microbial genetics but also have broader implications in biotechnology and medicine, where knowledge of DNA repair mechanisms can inform the development of genetic engineering techniques and therapies for genetic disorders. Thus, the exploration of RecO proteins represents a significant area of molecular biology research with implications that extend beyond basic science into the fields of health and disease management.
