Analytical Data
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基因名
SARS-CoV-2 RdRP 蛋白
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简介
The multifunctional SARS-CoV-2 PP1ab protein is essential for viral RNA transcription and replication, utilizing proteases for multi-protein cleavage. It inhibits host translation by binding to the 40S subunit and blocks ribosomal mRNA entry channels, thereby hindering the antiviral response. RdRP Protein, Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) (Sf9, His, Strep) is the recombinant virus-derived RdRP, expressed by Sf9 insect cells, with Strep, His labeled tag.
- Application
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种属
Virus
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表达系统
Baculovirus
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标签
N-Strep;N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0DTD1-1
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表达区间
S4393-Q5324
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蛋白长度
Partial
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of SARS-CoV-2 RNA-dependent RNA polymerase (RdRP) recombinant protein has become particularly significant in the context of the COVID-19 pandemic, which is caused by the novel coronavirus SARS-CoV-2. RdRP plays a crucial role in the viral replication cycle, catalyzing the synthesis of viral RNA from the RNA template, and thus is a prime target for antiviral drug development. Understanding the structure and function of RdRP is vital for the design of inhibitors that could effectively hinder the virus's ability to replicate. Since its emergence, researchers have focused on characterizing the RdRP protein at both molecular and structural levels to identify potential therapeutic compounds. Furthermore, recombinant RdRP has been utilized in various studies to elucidate the mechanism of RNA synthesis, aiding in the development of diagnostic tools and vaccines. The availability of the recombinant protein facilitates high-throughput screening of antiviral agents and helps in assessing their efficacy against SARS-CoV-2. As the virus mutates and new variants arise, ongoing research into the RdRP protein remains critical to stay ahead of viral threats and devise effective strategies for control and treatment.
