Analytical Data
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Gene name
GOT1
- Application
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Alternative Names
cCAT; AAT; ASAT; SGOT; GOT1; Cysteine transaminase, cytoplasmic; Transaminase A; Aspartate Transaminase 1,Cytoplasmic; Glutamic-Oxaloacetic Transaminase 1,Soluble
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P17174
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Expression Region
Met1~Gln413
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Molecular Weight
44kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GOT1, or Glutamate Oxidase Transaminase 1, is an enzyme that plays a crucial role in amino acid metabolism, particularly in the conversion of glutamate to α-ketoglutarate while simultaneously producing aspartate from oxaloacetate. The study of GOT1 recombinant protein has gained significant attention due to its potential implications in various fields, including metabolic disorders, neurobiology, and cancer research. Alterations in GOT1 activity have been linked to pathological conditions such as neurodegenerative diseases, where glutamate excitotoxicity contributes to neuronal damage. By producing recombinant GOT1, researchers aim to elucidate its structure-function relationships, understand its enzymatic mechanisms, and explore its interaction with other biomolecules. Additionally, recombinant GOT1 can serve as a valuable tool for drug discovery and the development of therapeutic strategies targeting metabolic pathways. The ability to produce large quantities of active GOT1 in vitro enables detailed kinetic studies and the exploration of its role in metabolic flux, ultimately contributing to our understanding of cellular metabolism and its dysregulation in disease states. Overall, research on GOT1 recombinant protein continues to expand our knowledge of its physiological functions and potential therapeutic applications, marking it as a promising target for future investigations in biochemistry and medicine.
