Analytical Data
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Gene name
SARS-CoV-2 NSP7
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简介
The multifunctional PL-PRO protein plays a critical role in viral RNA transcription and replication, providing the necessary proteases for multiprotein cleavage. It strategically blocks host translation by binding to the open head conformation of the 40S subunit, impeding ribosomal mRNA entry into the tunnel. SARS-CoV-2 NSP7 Protein (His-Avi) is the recombinant virus-derived NSP7, expressed by E. coli, with C-His-Avi labeled tag.
- Application
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Alternative Names
Replicase polyprotein 1a; ORF1a polyprotein; Severe acute respiratory syndrome coronavirus 2; 2019-nCoV; pp1a; Endonuclease; Hydrolase; Methyltransferase; Nuclease; Protease; RNA-binding; Thiol protease; Transferase
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Species
Virus
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Source
E. coli
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Tag
C-Avi;C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0DTC1-1
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Expression Region
S3860-Q3942
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The SARS-CoV-2 NSP7 (non-structural protein 7) is a critical component of the viral replication process and plays a significant role in the formation of the viral RNA-dependent RNA polymerase complex alongside NSP8 and NSP12. Understanding the structure and function of NSP7 is crucial for the development of therapeutic strategies against COVID-19. Research into NSP7 has revealed its involvement in stabilizing the RNA polymerase complex, which is essential for efficient viral RNA synthesis. Scientists have focused on characterizing the recombinant NSP7 protein to explore its properties, including its interaction with other viral proteins and its role in the virus's life cycle. This research not only deepens our understanding of SARS-CoV-2 biology but also assists in the identification of potential drug targets. Moreover, the recombinant NSP7 protein can be utilized in the development of serological assays and vaccines, contributing to global efforts in controlling the pandemic. Given the ongoing evolution of SARS-CoV-2 variants, studying NSP7 remains relevant for informing public health strategies and enhancing our ability to respond to emerging viral threats.
