Recombinant Streptococcus equi subsp. zooepidemicus ideZ Protein，His

IdeZ, an endopeptidase derived from the bacterium *Lactococcus lactis*, exhibits unique properties that make it a significant candidate for biotechnological and therapeutic applications. It specifically cleaves immunoglobulin G (IgG) molecules at a designated site, yielding functional Fab fragments that can be utilized in various biomedical applications, including antibody engineering and the development of new immunotherapies. The enzyme's specificity for IgG provides advantages in producing antibody fragments with retained antigen-binding capabilities while avoiding the potential immunogenicity associated with whole antibodies. The interest in IdeZ has grown substantially in the field of protein engineering, particularly due to its ability to help produce monoclonal antibodies and improve the efficacy of antibody-based drugs. Researchers have focused on optimizing the production and activity of IdeZ, exploring its structural characteristics to enhance its stability and reactivity under physiological conditions. Additionally, the enzyme's potential role in therapeutic settings, such as targeted drug delivery and cancer treatment, continues to drive investigations into its biochemical properties and mechanisms of action. Consequently, IdeZ represents a promising subject for future studies aimed at unraveling its full potential in both basic research and clinical applications.