Analytical Data
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Gene name
GGT1
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简介
The GGT1 protein cleaves gamma-glutamyl bonds of extracellular glutathione, glutathione conjugates, and other gamma-glutamyl compounds. Its metabolism releases free glutamate and cysteinylglycine, which are hydrolyzed into cysteine and glycine. GGT1 Protein, Human (HEK293, His) is the recombinant human-derived GGT1 protein, expressed by HEK293 , with N-His labeled tag.
- Application
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Alternative Names
Gamma-glutamyltransferase 1; GGT 1; CD224; GGT; GGT-1; GGTD; D22S672; D22S73; D22S732
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Species
Human
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Source
HEK293
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Tag
N-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P19440-1
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Expression Region
P27-Y569
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Protein Length
Extracellular Domain
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Molecular Weight
55-65 kDa (heavy chain) & 25-30 kDa (light chain)
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GGT1, or gamma-glutamyltransferase 1, is an enzyme pivotal in the metabolism of glutathione and the modulation of cellular oxidative stress. It plays a significant role in catalyzing the transfer of gamma-glutamyl groups from glutathione to various amino acids and peptides, thereby participating in pivotal cellular processes. Research indicates that GGT1 is implicated in a variety of physiological and pathological states, including tumor progression, liver diseases, and neurodegenerative disorders, where its expression levels often correlate with disease severity or patient prognosis. The interest in recombinant GGT1 protein arises from its potential as a therapeutic target and a biomarker for disease diagnosis. Understanding its structure and function through recombinant expression systems allows for the exploration of its enzymatic mechanisms and interactions. Furthermore, the availability of purified GGT1 protein provides a powerful tool for drug development, elucidation of metabolic pathways, and investigation of its role in oxidative stress responses. Studies in this domain aim to provide insights into how GGT1 modulation may contribute to therapeutic strategies in cancer treatment and oxidative stress management, highlighting its significance in both basic and applied biomedical research.
