Analytical Data
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基因名
ST3GAL4
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简介
The ST3GAL4 protein is a β-galactoside α2-3 sialyltransferase essential for the sialylation of glycoproteins and glycolipids. It mediates hemostasis by sialylating VWF, preventing ASGPR recognition and clearance, and regulating platelet clearance. ST3GAL4 Protein, Human (HEK293, His-HA) is the recombinant human-derived ST3GAL4 protein, expressed by HEK293 , with C-His, N-HA labeled tag.
- Application
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生物活性
1. Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to alpha –Lactose that incubate at 37°C for 30min .The specific activity is 499.44 pmol/min/μg. 2. Western blot analysis of protein Human ST3GAL4 (lane 2 (10ng) and lane 3 (20ng)) using HA Tag (HRP) (HY-P80948A) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) in 5% non-fat milk in TBST at 4°C overnight. Western blot analysis of protein Human ST3GAL4 (lane 2 (10ng) and lane 3 (20ng)) using HA Tag (HRP) (HY-P80948A) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) in 5% non-fat milk in TBST at 4°C overnight.
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别名
Sialyltransferase 4C; SIAT4; SIAT4C; SIAT4-C; ST3Gal IV; ST3GAL4; ST3GalA.2; ST-4; STZSIAT4; Alpha 2,3-ST 4; CGS23; CGS23FLJ46764; FLJ11867; Gal-NAc 6S; NANTA3; SAT3; SAT-3
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种属
Human
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表达系统
HEK293
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标签
C-8*His;N-HA
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q11206
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表达区间
E41-F333
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蛋白长度
Partial
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分子量
48-53 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ST3GAL4, a member of the sialyltransferase family, plays a crucial role in glycoprotein and glycolipid sialylation, which is fundamental for various biological processes, including cell-cell communication, immune response, and tumor progression. As an enzyme that transfers sialic acid from the donor substrate CMP-sialic acid to glycan acceptors, ST3GAL4 specifically catalyzes the formation of α-2,3-sialylated glycotopes. This post-translational modification influences not only the physiological functions of proteins but also their stability and recognition by different receptors. Aberrant expression of ST3GAL4 has been implicated in various diseases, particularly cancers, where altered sialylation patterns can enhance tumor aggressiveness and metastasis. Research into the characterization and functional analysis of recombinant ST3GAL4 protein not only provides insights into its enzymatic activity but also reveals its potential as a therapeutic target for diseases characterized by dysregulated sialylation. Understanding the mechanistic basis of ST3GAL4's function can help develop sialylation inhibitors or modulating agents, aiming to improve treatment strategies for cancer and other sialylation-related disorders.
