Analytical Data
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基因名
PFKM
- Application
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别名
PFK1; PFKX; Phosphofructokinase 1; Phosphofructo-1-kinase isozyme A; 6-Phosphofructokinase Polypeptide X; Fructose-6-Phosphate 1-Phosphotransferase
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种属
Rat
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P47858
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表达区间
Thr2~His390
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分子量
46kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Phosphofructokinase M (PFKM) is a key regulatory enzyme in the glycolytic pathway, responsible for converting fructose-6-phosphate into fructose-1,6-bisphosphate, a critical step in glucose metabolism. The PFKM enzyme is primarily expressed in muscle and brain tissues, playing an essential role in energy production, cellular respiration, and metabolic regulation. Alterations in PFKM activity have been associated with various metabolic disorders, including obesity, diabetes, and certain types of cancers, highlighting its significance in both physiological and pathological contexts. Recent studies have focused on the recombinant expression of PFKM in heterologous systems, enabling researchers to investigate its structure, function, and regulation under different conditions. Such studies aim to provide insights into the enzyme's kinetic properties and its role in metabolic pathways. Additionally, recombinant PFKM can serve as a valuable tool in drug discovery and the development of therapeutic interventions targeting metabolic diseases. Understanding the biochemical properties of this enzyme at the molecular level is crucial for elucidating its role in health and disease, paving the way for novel treatment strategies aimed at modulating glycolytic flux and energy metabolism in affected tissues.
