Analytical Data
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Gene name
Hemoglobin subunit zeta/HBAZ
- Application
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Alternative Names
HBAZ Hemoglobin zeta chain Zeta-globin
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Species
Human
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Source
E. coli
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Tag
N- GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P02008
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Expression Region
1-142aa
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Molecular Weight
42.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Hemoglobin subunit zeta (HBAZ) is a member of the globin gene family, predominantly expressed during embryonic development, particularly in the yolk sac and early stages of erythropoiesis. Hemoglobin is critical for oxygen transport in vertebrates, and the zeta subunit plays a vital role in the formation of embryonic hemoglobin, known as embryonic hemoglobin (HbE), which consists of two zeta and two epsilon chains. The study of HBAZ recombinant protein is significant for understanding the molecular mechanisms underlying hemoglobin synthesis and its regulatory pathways during development. In various model organisms, the expression of zeta chains has been linked to lineage commitment in erythropoiesis, shedding light on how embryonic blood cells differentiate from progenitor cells. Additionally, mutations or dysregulation in hemoglobin genes, including those encoding the zeta chain, can lead to hematological disorders such as thalassemia and other anemias. By producing and characterizing HBAZ recombinant protein, researchers aim to investigate its functional role in hemoglobin structure and stability, as well as its interactions with other globin chains. This research not only enhances the understanding of normal hemoglobin development but also has potential therapeutic implications for treating disorders associated with abnormal hemoglobin function. The recombinant HBAZ protein can serve as a valuable tool in both basic and applied research, contributing to advancements in regenerative medicine and genetic therapies targeting hemoglobinopathies.
