Analytical Data
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基因名
Thymidylate
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简介
Thymidylate proteins play a key role in DNA synthesis by catalyzing the phosphorylation of thymidine monophosphate (dTMP) to thymidine diphosphate (dTDP). This enzymatic process is critical for the production of dTDP, the direct precursor of the DNA building block dTTP. Thymidylate Protein, Human (HEK293, His) is the recombinant human-derived Thymidylate protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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别名
rHuThymidylate /kinaseDTYMK, His; Thymidylate kinase; dTMP kinase; DTYMK; CDC8; TMPK; TYMK
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P23919
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表达区间
M1-K212
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氨基酸序列
MAARRGALIVLEGVDRAGKSTQSRKLVEALCAAGHRAELLRFPERSTEIGKLLSSYLQKKSDVEDHSVHLLFSANRWEQVPLIKEKLSQGVTLVVDRYAFSGVAFTGAKENFSLDWCKQPDVGLPKPDLVLFLQLQLADAAKRGAFGHERYENGAFQERALRCFHQLMKDTTLNWKMVDASKSIEAVHEDIRVLSEDAIRTATEKPLGELWK
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蛋白长度
Full Length
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分子量
26.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Thymidylate synthase (TS) is a vital enzyme involved in the de novo synthesis of thymidine, which is essential for DNA replication and repair. TS catalyzes the reductive methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) using tetrahydrofolate as a cofactor. Given its crucial role in the synthesis of DNA, TS has garnered significant attention in cancer research, particularly because its inhibition can lead to the depletion of nucleotide pools, thereby hindering tumor cell proliferation. Several chemotherapeutic agents, such as 5-fluorouracil, target TS, and understanding the structure and function of the enzyme can provide insights into drug resistance mechanisms and facilitate the design of novel therapeutic strategies. Moreover, recombinant TS proteins have been produced for detailed biochemical and structural studies, allowing researchers to uncover the enzyme's mechanism of action, substrate specificity, and interactions with inhibitors. These studies not only enhance the understanding of TS's role in cellular processes but also hold potential for developing targeted cancer therapies. The continuous exploration of TS and its recombinant forms stands as a cornerstone in the search for effective treatments against malignancies characterized by dysregulated DNA synthesis.
