Analytical Data
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Gene name
ALK-7
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简介
ALK-7 is a type I receptor serine-threonine kinase mediate inhibitory as well as stimulatory signals for growth and differentiation by binding to members of the TGF-β superfamily. ALK-7 combined with specific ligands, such as Nodal, activin B and growth differentiation factor (GDF), can activate Smads and other signaling pathways, thereby regulating cell proliferation, differentiation and apoptosis in various cells[1][2][3]. ALK-7 Protein, Human (HEK293, Fc) is produced in HEK293 cells with a C-Terminal Fc-tag. It consists of 113 amino acids (M1-E113).
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human ALK-7 is immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated Recombinant Human Nodal. The ED50 for this effect is 243.4 ng/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human ALK-7 is immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated Recombinant Human Nodal. The ED50 for this effect is 243.4 ng/mL.
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Alternative Names
Activin receptor type IC; ACTR-IC; ACVRLK7; ALK7
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Species
Human
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
Q8NER5
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Expression Region
L22-E113
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Protein Length
Extracellular Domain
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Molecular Weight
The recombinant human ALK-7 consists of 92 amino acids & hFc tag and predicts a molecular mass of 35.96 kDa. It migrates as an 43-56 kDa band in SDS-PAGE under reducing conditions.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ALK-7, also known as activin receptor-like kinase 7, is a member of the transforming growth factor-beta (TGF-β) superfamily and plays a crucial role in various physiological and pathological processes, including neuronal development, pain modulation, and metabolism. The interest in ALK-7 has grown due to its involvement in the regulation of energy homeostasis and its potential links to obesity and metabolic disorders. Studies have shown that ALK-7 mediates some of the effects of the appetite-regulating hormone, leptin, and is expressed in specific brain regions associated with energy balance. Research into ALK-7 recombinant proteins aims to elucidate its signaling pathways and biological functions, which could lead to novel therapeutic strategies for metabolic diseases and inflammatory conditions. Furthermore, understanding how ALK-7 interacts with other receptors and signaling molecules could provide insights into neuroendocrine pathways and their implications for health and disease. In recent years, advances in recombinant protein technology have facilitated the production and characterization of ALK-7, allowing researchers to investigate its mechanisms of action in greater detail, paving the way for potential applications in drug development and disease treatment.
