Analytical Data
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Gene name
MGAT5
- Application
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Biological Activity
Measured in a cell proliferation assay using MDA-MB-231 cells. The ED50 for this effect is <0>1×10^4 units/mg. Measured in a cell proliferation assay using MDA-MB-231 cells. The ED50 for this effect is 0.01096 μg/mL, corresponding to a specific activity is 9.124×104 units/mg.
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Alternative Names
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; GNT-V; Secreted GNT-V; MGAT5; GGNT5
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Species
Human
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Source
HEK293
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Tag
C-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q09328
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Expression Region
L189-L741
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AA Sequence
LSEVENWCPHLPWRAKNPYEEADHNSLAEIRTDFNILYSMMKKHEEFRWMRLRIRRMADAWIQAIKSLAEKQNLEKRKRKKVLVHLGLLTKESGFKIAETAFSGGPLGELVQWSDLITSLYLLGHDIRISASLAELKEIMKKVVGNRSGCPTVGDRIVELIYIDIVGLAQFKKTLGPSWVHYQCMLRVLDSFGTEPEFNHANYAQSKGHKTPWGKWNLNPQQFYTMFPHTPDNSFLGFVVEQHLNSSDIHHINEIKRQNQSLVYGKVDSFWKNKKIYLDIIHTYMEVHATVYGSSTKNIPSYVKNHGILSGRDLQFLLRETKLFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRELTSQHPYAEVFIGRPHVWTVDLNNQEEVEDAVKAILNQKIEPYMPYEFTCEGMLQRINAFIEKQDFCHGQVMWPPLSALQVKLAEPGQSCKQVCQESQLICEPSFFQHLNKDKDMLKYKVTCQSSELAKDILVPSFDPKNKHCVFQGDLLLFSCAGAHPRHQRVCPCRDFIKGQVALCKDCL
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Protein Length
Partial
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Molecular Weight
72-78 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MGAT5, or mannosyltransferase 5, is an essential enzyme involved in the glycosylation process, particularly in the synthesis of complex N-glycans that are vital for proper protein folding and stability. Dysregulation of MGAT5 has been implicated in various diseases, including cancer progression and metastasis, as it influences cell signaling pathways and interactions with the extracellular matrix. The study of MGAT5 recombinant proteins is therefore crucial for understanding its biochemical properties, enzymatic activity, and role in glycan biosynthesis. By engineering MGAT5 in recombinant systems, researchers can elucidate its structure-function relationships and the effects of glycan modifications on cell behavior. This research not only aims to develop potential therapeutic strategies targeting MGAT5 but also provides insights into the broader implications of glycosylation in health and disease. As such, MGAT5 stands as a significant target in glycoscience and therapeutic development, warranting extensive investigation into its recombinant form for potential applications in biotechnology and medicine.
