Analytical Data
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Gene name
SLC12A1
- Application
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Alternative Names
SLC12A1; NKCC2; Solute Carrier Family 12 Member 1; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; Kidney-specific Na-K-Cl symporter
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q13621
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Expression Region
Arg814~Ser1099
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Molecular Weight
39kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SLC12A3, also known as the thiazide-sensitive Na-Cl cotransporter, is a critical membrane protein primarily expressed in the distal convoluted tubule of the kidney, where it plays a vital role in the reabsorption of sodium and chloride ions. Mutations in the SLC12A3 gene are associated with the genetic disorder Gitelman syndrome, which is characterized by electrolyte imbalances such as hypomagnesemia and hypocalciuria, leading to muscle cramps, weakness, and fatigue. Research on SLC12A3 has gained significant attention due to its essential functions in maintaining electrolyte homeostasis and its implications in nephrology. The study of recombinant SLC12A3 protein is crucial for understanding its structure-function relationship, regulatory mechanisms, and interaction with various ligands and medications, particularly thiazide diuretics. By producing and characterizing this recombinant protein, researchers aim to elucidate the molecular bases of SLC12A3 function and dysfunction, providing insights into potential therapeutic targets for managing electrolyte disorders and related pathologies.
