Analytical Data
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基因名
DSPG3/Epiphycan
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简介
DSPG3/Epiphycan Protein plays a crucial role in bone formation and the establishment of ordered cartilage structure by contributing to matrix organization. Its multifaceted function in skeletal development influences dynamic processes related to bone formation and cartilage structural integrity. The protein's dual impact underscores its significance in maintaining the integrity and organization of the extracellular matrix in skeletal tissues. DSPG3/Epiphycan Protein, Human (HEK293, His) is the recombinant human-derived DSPG3/Epiphycan protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
Measured by the ability of the immobilized protein to enhance the adhesion of Saos-2 human osteosarcoma cells to human Fibronectin. The ED50 for this effect is 0.1403 μg/mL in the presence of 0.5 μg/mL Fibronectin, corresponding to a specific activity is 7.127×10^3 units/mg. Measured by the ability of the immobilized protein to enhance the adhesion of Saos-2 human osteosarcoma cells to human Fibronectin. The ED50 for this effect is 0.1403 μg/mL in the presence of 0.5 μg/mL Fibronectin, corresponding to a specific activity is 7.127×10^3 units/mg.
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别名
Dermatan sulfate proteoglycan 3; Proteoglycan-Lb; EPYC; PGLB; SLRR3B
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种属
Human
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q99645
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表达区间
A20-V322
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氨基酸序列
APTLESINYDSETYDATLEDLDNLYNYENIPVDKVEIEIATVMPSGNRELLTPPPQPEKAQEEEEEEESTPRLIDGSSPQEPEFTGVLGPHTNEDFPTCLLCTCISTTVYCDDHELDAIPPLPKNTAYFYSRFNRIKKINKNDFASLSDLKRIDLTSNLISEIDEDAFRKLPQLRELVLRDNKIRQLPELPTTLTFIDISNNRLGRKGIKQEAFKDMYDLHHLYLTDNNLDHIPLPLPENLRALHLQNNNILEMHEDTFCNVKNLTYIRKALEDIRLDGNPINLSKTPQAYMCLPRLPVGSLV
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蛋白长度
Full Length of Mature Protein
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分子量
43-52 kDa.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DSPG3, or Dermatan Sulfate Proteoglycan 3, is a member of the small leucine-rich proteoglycan (SLRP) family, which plays a significant role in the extracellular matrix (ECM) dynamics and cellular interactions. Epiphycan, a closely related member of this family, is known for its involvement in cartilage and bone development, as well as its regulatory roles in cell proliferation and differentiation. Recent studies have highlighted the importance of DSPG3 in various physiological and pathological processes, including tissue repair and inflammatory responses. The recombinant expression of DSPG3 and Epiphycan proteins has garnered attention, as it allows for the exploration of their functional properties, interactions with other ECM components, and potential therapeutic applications in conditions like osteoarthritis and degenerative joint diseases. By producing and characterizing these recombinant proteins, researchers aim to better understand their specific roles in cellular signaling pathways and ECM organization and to develop new strategies for regenerative medicine and tissue engineering. This research not only illuminates the fundamental biological mechanisms but also holds promise for innovative treatments for musculoskeletal disorders.
