Analytical Data
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基因名
Heparanase/HPSE
- Application
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生物活性
1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet. 2.Measured by its binding ability in a functional ELISA. Immobilized HPSE at 2 μg/ml (100μl/well) can bind HPSE antibody. The ED50 for this effect is 0.2357 μg/mL. Measured by its binding ability in a functional ELISA. Immobilized HPSE at 2 μg/ml (100μl/well) can bind HPSE antibody. The ED50 for this effect is 0.2357 μg/mL.
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别名
Heparanase; Endo-glucoronidase; Heparanase-1; Hpa1; HEP, HPA, HPA1, HPR1, HPSE1, HSE1; HPSE
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种属
Human
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表达系统
CHO
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标签
N-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
AAD41342.1
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表达区间
Q36-I543
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蛋白长度
Full length of Mature Protein
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分子量
78 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
Heparanase (HPSE) is an endo-β-glucuronidase enzyme responsible for the degradation of heparan sulfate, a key component of the extracellular matrix and cell surface proteoglycans. It plays a crucial role in various physiological and pathological processes, including tumor progression, angiogenesis, and inflammation. Elevated HPSE levels have been associated with aggressive cancer phenotypes, making it a potential therapeutic target for cancer treatment. Research into recombinant HPSE has gained significant attention due to its potential applications in understanding the enzyme's biological functions and developing new therapeutic strategies. Recombinant HPSE can be produced using various expression systems, enabling studies on its enzymatic activity, substrate specificity, and interaction with other biomolecules. Moreover, insights gained from recombinant protein studies can aid in designing HPSE inhibitors, which could serve as novel anti-cancer agents. The exploration of HPSE's role in the tumor microenvironment and its implications in metastasis further highlights its significance in cancer research, necessitating an ongoing investigation into its molecular mechanisms and potential as a biomarker for cancer prognosis. Overall, the study of recombinant heparanase is critical for devising innovative therapeutic approaches to target malignancies and improve patient outcomes.
