Analytical Data
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Gene name
SLC12A2
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简介
SLC12A2 Protein mediates electroneutral transport of chloride, potassium, and/or sodium ions across the membrane, crucial for regulating ionic balance and cell volume. SLC12A2 Protein, Human (HEK293, His, Strep, GFP) is the recombinant human-derived SLC12A2 protein, expressed by HEK293 , with N-Strep, N-EGF, N-His labeled tag.
- Application
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Alternative Names
SLC12A2; Solute carrier family 12 member 2; Basolateral Na-K-Cl symporter; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2
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Species
Human
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Source
HEK293
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Tag
N-StrepⅡ;N-His;N-GFP
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P55011-1
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Expression Region
M1-S1212
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Protein Length
Full Length of Isoform-1
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SLC12A2, also known as the solute carrier family 12 member 2, is a key protein involved in sodium-coupled transport processes in epithelial cells, particularly in the renal and intestinal systems. Its primary role is to facilitate the reabsorption of sodium and chloride ions, which is crucial for maintaining electrolyte balance, blood pressure regulation, and overall fluid homeostasis. Dysregulation or mutations in SLC12A2 have been implicated in various pathological conditions, including hypertension, metabolic disorders, and electrolyte imbalances. Research on recombinant SLC12A2 proteins has gained traction as scientists aim to elucidate the structural and functional dynamics of this transporter. By producing and studying SLC12A2 in vitro, researchers can analyze its interaction with different substrates and inhibitors, gain insights into its transport mechanism, and explore its role in health and disease. The generation of high-quality recombinant SLC12A2 is imperative for advancing our understanding of its physiological functions and developing potential therapeutic strategies targeting related disorders. Moreover, this research contributes to the broader understanding of ion transporters and their significance in cellular homeostasis, paving the way for innovations in drug development and personalized medicine.
