Analytical Data
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Gene name
GBP4
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简介
GBP4, an IFN-inducible GTPase, crucially defends against various pathogens.It hydrolyzes GTP to GDP and aids inflammasome assembly by releasing ligands from bacteria.GBP4 promotes pathogen-containing vacuole lysis, facilitating cytosolic entry, where it recruits proteins like Gm12250/Irgb10 for bacterial cytolysis.This liberation activates inflammasomes, including CASP4/CASP11 and AIM2.GBP4 may also influence erythroid differentiation.GBP4 protein, mouse (HEK293, His) is the recombinant mouse-derived GBP4 protein, expressed by HEK293 , with N-8*His labeled tag.
- Application
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Alternative Names
GBP4; guanylate binding protein 4; guanylate-binding protein 4; Mpa2; GBP-4; GTP-binding protein 4; guanine nucleotide-binding protein 4
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Species
Mouse
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Source
HEK293
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Tag
N-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q61107
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Expression Region
E2-S620
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AA Sequence
EAPICLVENWKNQLTVNLEAIRILEQIAQPLVVVAIVGLYRTGKSYLMNRLAGRNHGFSLGSTVQSETKGIWMWCVPHPTKPTHTLVLLDTEGLGDVEKGDPKNDSWIFALAVLLSSTFVYNSMSTINQQALEQLHFVTELTQLIRAKSSPREDKVKDSSEFVGFFPDFIWAVRDFALELKLNGRPITEDEYLENALKLIQGDNLKVQQSNMTRECIRYFFPVRKCFVFDRPTSDKRLLLQIENVPENQLERNFQVESEKFCSYIFTNGKTKTLRGGVIVTGNRLGTLVQTYVNAINSGTVPCLENAVTTLAQRENSIAVQKAADHYSEQMAQRMRLPTDTLQELLTVHAACEKEAIAVFMEHSFKDDEQEFQKKLVVTIEERKEEFIRQNEAASIRHCQAELERLSESLRKSISCGAFSVPGGHSLYLEARKKIELGYQQVLRKGVKAKEVLKSFLQSQAIMEDSILQSDKALTDGERAIAAERTKKEVAEKELELLRQRQKEQEQVMEAQERSFRENIAKLQEKMESEKEMLLREQEKMLEHKLKVQEELLIEGFREKSDMLKNEISHLREEMERTRRKPSLFGQILDTIGNAFIMILPGAGKLFGVGLKFLGSLSS
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Protein Length
Partial
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Molecular Weight
73.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GBP4, or Guanylate-Binding Protein 4, is a member of the GBP family, which plays a crucial role in the innate immune response to viral and bacterial infections. These proteins are induced by interferons and exhibit GTPase activity, enabling them to modulate various cellular processes. GBP4 has garnered attention for its involvement in host defense mechanisms, including the regulation of inflammatory responses and apoptosis. Recent studies have suggested that GBP4 may also have a role in cancer biology, influencing tumor progression and response to therapies. The understanding of GBP4's structure and function could provide insights into its mechanisms of action and potential therapeutic applications. Moreover, as researchers explore GBP4's interactions with other proteins and its impact on cellular pathways, there is growing interest in harnessing its properties for developing innovative treatments, including targeted therapies and immunomodulators. By elucidating the molecular mechanisms underlying GBP4's activities, scientists aim to unveil its full potential in combating infections and diseases, making it a significant focus within the field of immunology and molecular biology.
