Analytical Data
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Gene name
NPR1
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简介
NPR1 Protein, a receptor for atrial natriuretic peptide NPPA/ANP and brain natriuretic peptide NPPB/BNP, initiates signaling cascades with guanylate cyclase activity upon ligand binding. Essential vasoactive hormones, these peptides regulate cardiovascular homeostasis. NPR1 significantly contributes to vascular tone and fluid balance regulation in the cardiovascular system through its role as a receptor for these natriuretic peptides. NPR1 Protein, Human (HEK293, His) is the recombinant human-derived NPR1 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Alternative Names
ANP-A; ANPa; Atrionatriuretic peptide receptor A; Guanylate cyclase A; Guanylate cyclase; GUC2A; NPRA
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Species
Human
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Source
HEK293
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Tag
C-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P16066
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Expression Region
G33-E473
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AA Sequence
GNLTVAVVLPLANTSYPWSWARVGPAVELALAQVKARPDLLPGWTVRTVLGSSENALGVCSDTAAPLAAVDLKWEHNPAVFLGPGCVYAAAPVGRFTAHWRVPLLTAGAPALGFGVKDEYALTTRAGPSYAKLGDFVAALHRRLGWERQALMLYAYRPGDEEHCFFLVEGLFMRVRDRLNITVDHLEFAEDDLSHYTRLLRTMPRKGRVIYICSSPDAFRTLMLLALEAGLCGEDYVFFHLDIFGQSLQGGQGPAPRRPWERGDGQDVSARQAFQAAKIITYKDPDNPEYLEFLKQLKHLAYEQFNFTMEDGLVNTIPASFHDGLLLYIQAVTETLAHGGTVTDGENITQRMWNRSFQGVTGYLKIDSSGDRETDFSLWDMDPENGAFRVVLNYNGTSQELVAVSGRKLNWPLGYPPPDIPKCGFDNEDPACNQDHLSTLE
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Protein Length
Extracellular Domain
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Molecular Weight
60-75 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NPR1 (NPR1-like protein 1) is a key regulatory protein in plants, particularly known for its role in mediating systemic acquired resistance (SAR) and plant immune responses. Research into NPR1 has gained prominence due to its central function in plant defense mechanisms against pathogens, particularly during systemic signaling processes. NPR1 operates as a molecular switch, activating various defense-related genes in response to pathogen attack and environmental stressors. It is also involved in the crosstalk between different plant signaling pathways, integrating salicylic acid (SA) signaling with those of other phytohormones, which is critical for fine-tuning immune responses. The study of NPR1 and its recombinant forms has implications for enhancing plant resistance to diseases, improving crop yield and quality. Furthermore, understanding the structure-function relationships of NPR1 can provide insights into its activation mechanisms, potentially leading to biotechnological applications aimed at engineering disease-resistant crops. Given its pivotal role in plant immunity, NPR1 is not only a subject of basic research but also a target for agricultural innovations aimed at sustainable food production in the face of increasing biotic challenges.
