Analytical Data
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基因名
Alpha-2-macroglobulin
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简介
Alpha-2-macroglobulin Protein, a protease inhibitor and cytokine transporter, belongs to the MEROPS proteinase inhibitor family I39, clan IL. A2M acts on all classes of endoproteases and could potentially be used to slow or halt PTOA by neutralizing catabolic cascades in affected cartilage. A2M can enter into cells and bind to numerous cytokines. It inhibits inflammatory mediators of chondrocytes by blocking IL-1尾/NF-魏B pathway. Alpha-2-macroglobulin Protein, Human (sf9, His) is the recombinant human-derived Alpha-2-macroglobulin protein, expressed by Sf9 insect cells , with C-His labeled tag.
- Application
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别名
Alpha-2-macroglobulin; Alpha-2-M; A2M; CPAMD5
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种属
Human
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表达系统
Baculovirus
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标签
C-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
NP_000005.2
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表达区间
S24-A1474
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氨基酸序列
SVSGKPQYMVLVPSLLHTETTEKGCVLLSYLNETVTVSASLESVRGNRSLFTDLEAENDVLHCVAFAVPKSSSNEEVMFLTVQVKGPTQEFKKRTTVMVKNEDSLVFVQTDKSIYKPGQTVKFRVVSMDENFHPLNELIPLVYIQDPKGNRIAQWQSFQLEGGLKQFSFPLSSEPFQGSYKVVVQKKSGGRTEHPFTVEEFVLPKFEVQVTVPKIITILEEEMNVSVCGLYTYGKPVPGHVTVSICRKYSDASDCHGEDSQAFCEKFSGQLNSHGCFYQQVKTKVFQLKRKEYEMKLHTEAQIQEEGTVVELTGRQSSEITRTITKLSFVKVDSHFRQGIPFFGQVRLVDGKGVPIPNKVIFIRGNEANYYSNATTDEHGLVQFSINTTNVMGTSLTVRVNYKDRSPCYGYQWVSEEHEEAHHTAYLVFSPSKSFVHLEPMSHELPCGHTQTVQAHYILNGGTLLGLKKLSFYYLIMAKGGIVRTGTHGLLVKQEDMKGHFSISIPVKSDIAPVARLLIYAVLPTGDVIGDSAKYDVENCLANKVDLSFSPSQSLPASHAHLRVTAAPQSVCALRAVDQSVLLMKPDAELSASSVYNLLPEKDLTGFPGPLNDQDDEDCINRHNVYINGITYTPVSSTNEKDMYSFLEDMGLKAFTNSKIRKPKMCPQLQQYEMHGPEGLRVGFYESDVMGRGHARLVHVEEPHTETVRKYFPETWIWDLVVVNSAGVAEVGVTVPDTITEWKAGAFCLSEDAGLGISSTASLRAFQPFFVELTMPYSVIRGEAFTLKATVLNYLPKCIRVSVQLEASPAFLAVPVEKEQAPHCICANGRQTVSWAVTPKSLGNVNFTVSAEALESQELCGTEVPSVPEHGRKDTVIKPLLVEPEGLEKETTFNSLLCPSGGEVSEELSLKLPPNVVEESARASVSVLGDILGSAMQNTQNLLQMPYGCGEQNMVLFAPNIYVLDYLNETQQLTPEIKSKAIGYLNTGYQRQLNYKHYDGSYSTFGERYGRNQGNTWLTAFVLKTFAQARAYIFIDEAHITQALIWLSQRQKDNGCFRSSGSLLNNAIKGGVEDEVTLSAYITIALLEIPLTVTHPVVRNALFCLESAWKTAQEGDHGSHVYTKALLAYAFALAGNQDKRKEVLKSLNEEAVKKDNSVHWERPQKPKAPVGHFYEPQAPSAEVEMTSYVLLAYLTAQPAPTSEDLTSATNIVKWITKQQNAQGGFSSTQDTVVALHALSKYGAATFTRTGKAAQVTIQSSGTFSSKFQVDNNNRLLLQQVSLPELPGEYSMKVTGEGCVYLQTSLKYNILPEKEEFPFALGVQTLPQTCDEPKAHTSFQISLSVSYTGSRSASNMAIVDVKMVSGFIPLKPTVKMLERSNHVSRTEVSSNHVLIYLDKVSNQTLSLFFTVLQDVPVRDLKPAIVKVYDYYETDEFAIAEYNAPCSKDLGNA
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蛋白长度
Full Length of Mature Protein
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分子量
160-170 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Amyloid precursor protein (APP), particularly the isoform APP-751, has garnered significant attention in Alzheimer's disease research due to its pivotal role in the generation of amyloid-beta peptides, which aggregate to form plaques—a hallmark of the disease. APP is a transmembrane protein predominantly expressed in neurons, and its normal physiological functions are linked to neuronal growth, survival, and synaptic repair. However, abnormal processing of APP, particularly through the amyloidogenic pathway, results in the production of neurotoxic amyloid-beta. Understanding the structure and function of the APP-751 isoform is crucial, as its unique properties may influence the pathways leading to amyloid plaque formation and, consequently, neurodegeneration. Additionally, recombinant techniques to produce APP-751 protein enable detailed studies of its interactions, processing, and effects on cellular models. Research on APP-751 not only provides insights into the pathogenesis of Alzheimer's disease but also aids in the development of potential therapeutic strategies aimed at inhibiting amyloid-beta production and promoting healthy APP metabolism. By elucidating these mechanisms, we can advance our understanding of Alzheimer's disease etiology and identify new avenues for intervention to combat this devastating disorder.
