Analytical Data
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基因名
PDE4D
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简介
PDE4D (Phosphodiesterase 4D) protein, as a hydrolytic enzyme, degrades the second messenger cAMP, a crucial regulator in various physiological processes. Its enzymatic activity in breaking down cAMP underscores PDE4D's pivotal role in modulating cellular signaling cascades, maintaining the intricate balance of intracellular communication mediated by this critical second messenger. PDE4D Protein, Human (His) is the recombinant human-derived PDE4D protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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别名
PDE4D; cAMP-specific 3'; 5'-cyclic phosphodiesterase 4D; DPDE3; PDE43
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q08499
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表达区间
T388-S715
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蛋白长度
Partial
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分子量
40 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PDE4D (phosphodiesterase 4D) is an enzyme that plays a crucial role in the regulation of intracellular signaling by hydrolyzing cyclic adenosine monophosphate (cAMP), which is a key second messenger involved in various physiological processes, including cellular growth, differentiation, and inflammatory responses. The importance of PDE4D has gained attention due to its involvement in several pathological conditions such as asthma, chronic obstructive pulmonary disease (COPD), and neurological disorders. Research has shown that dysregulation of PDE4D activity can lead to heightened inflammation and impaired cellular signaling, making it a potential therapeutic target. The recombinant protein of PDE4D is particularly valuable for studying its enzymatic properties, interaction with other signaling molecules, and role in disease mechanisms. It can be produced using various expression systems, allowing for large-scale production to facilitate biochemical assays and drug screening. Additionally, understanding the structure-function relationship of PDE4D through recombinant forms can aid in the design of selective inhibitors, which may serve as effective treatments for conditions associated with abnormal PDE4D activity. The ongoing research on PDE4D recombinant protein thus holds promise for developing novel therapeutic strategies to mitigate the impact of diseases linked to cAMP regulation and offers insights into the enzyme's multifaceted roles in cellular processes.
