Analytical Data
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基因名
MBP
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简介
MBP proteins, especially the classical isoforms 4-13, dominate myelin membranes in the central nervous system and are critical for myelination and stability. In contrast, the non-canonical isoform 1-3/Golli-MBP may be involved in early brain development and contribute to transcriptional complexes that influence multiple cellular processes. MBP Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived MBP protein, expressed by P. pastoris , with N-6*His labeled tag.
- Application
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别名
MBP; Hmbpr; MGC99675; mld; Myelin A1 ; myelin basic; shi
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种属
Mouse
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表达系统
P. pastoris
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P04370
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表达区间
M1-R250
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蛋白长度
Full Length
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MBP (Maltose Binding Protein) is a widely used fusion tag in protein expression and purification, known for its ability to enhance solubility and facilitate the isolation of recombinant proteins. It originates from the Escherichia coli maltose operon and possesses a high affinity for maltose, making it an effective tool for chromatography applications. The research on MBP fusion proteins has gained momentum due to their versatility across various fields, including structural biology, enzymology, and therapeutic protein development. Scientists have leveraged MBP to improve the yields of otherwise challenging-to-express proteins, often resulting in properly folded and functional variants. Additionally, the use of MBP allows for the simplification of downstream purification processes, as these fusion proteins can be easily separated from host cell contaminants. Advances in MBP technology, including site-directed mutagenesis and novel purification methods, continue to enhance its utility. Recent studies have also explored the effects of MBP on protein stability and activity, providing insights into optimizing fusion tag systems for diverse applications. As research progresses, the understanding of MBP's role in protein engineering will further bolster its application in biotechnology and pharmaceutical industries, enabling the development of more effective diagnostic tools and therapeutic agents.
