Analytical Data
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Gene name
IL-4R alpha
- Application
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Biological Activity
Measured by its ability to inhibit IL-4-dependent proliferation of TF 1 human erythroleukemic cells. The ED50 for this effect is ≤18.24 ng/mL in the presence 0.2 ng/mL of Recombinant Human IL-4, corresponding to a specific activity is ≥5.482×104 units/mg. Measured by its ability to inhibit IL-4-dependent proliferation of TF 1 human erythroleukemic cells. The ED50 for this effect is 18.24 ng/mL in the presence 0.2 ng/mL of Recombinant Human IL‑4, corresponding to a specific activity is 5.482×104 units/mg.
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Alternative Names
Interleukin-4 receptor subunit alpha; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; CD124
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Species
Canine
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
XP_022275636.1
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Expression Region
G2-Q230
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AA Sequence
GRLCSGLTFPVSCLVLVWVASSGSVKVLHEPSCFSDYISTSVCQWKMDHPTNCSAELRLSYQLDFMGSENHTCVPENREDSVCVCSMPIDDAVEADVYQLDLWAGQQLLWSGSFQPSKHVKPRTPGNLTVHPNISHTWLLMWTNPYPTENHLHSELTYMVNVSNDNDPEDFKVYNVTYMGPTLRLAASTLKSGASYSARVRAWAQTYNSTWSDWSPSTTWLNYYEPWEQ
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Protein Length
Partial
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Molecular Weight
40-60 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Interleukin-4 receptor alpha (IL-4Rα) is a critical component of the immune system, primarily involved in mediating the effects of interleukin-4 (IL-4) and interleukin-13 (IL-13), two cytokines that play vital roles in regulating immune responses, inflammation, and the differentiation of helper T cells. Dysregulation of IL-4Rα signaling has been implicated in various allergic diseases, such as asthma, and other conditions like autoimmune disorders and certain cancers, making it a potential therapeutic target. Research into recombinant IL-4Rα proteins has gained traction as scientists explore their utility in both basic research and potential clinical applications. These recombinant proteins can be used to study the receptor's structure-function relationship, signaling pathways, and interactions with its ligands. Additionally, they offer a platform for developing therapeutic agents that can either block or enhance IL-4Rα signaling, providing avenues for novel treatments for diseases characterized by abnormal immune responses. Understanding the biology of IL-4Rα through recombinantly expressed proteins could ultimately lead to improved interventions for conditions mediated by dysregulated IL-4 and IL-13 signaling.
