Analytical Data
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基因名
CIAO1
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简介
CIAO1 protein is a key player in the cytosolic iron-sulfur protein assembly (CIA) complex, interacting with CIAO2A or CIAO2B and MMS19 to affect different iron-sulfur protein assembly pathways. The CIAO1:CIAO2B:MMS19 assembly facilitates most cytoplasmic and nuclear Fe/S proteins, while the CIAO1:CIAO2A complex contributes to ACO1 maturation and IREB2 stabilization. CIAO1 Protein, Human is the recombinant human-derived CIAO1 protein, expressed by E. coli , with tag free.
- Application
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别名
CIAO1; Probable cytosolic iron-sulfur protein assembly protein CIAO1; WD repeat-containing protein 39
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种属
Human
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表达系统
E. coli
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标签
Tag Free
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O76071
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表达区间
M1-L339
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蛋白长度
Full Length
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CIAO1 (Chloroplast Import Associated 1) is a protein that plays a crucial role in the import of proteins into chloroplasts, the organelles responsible for photosynthesis in plant cells. Research on CIAO1 has gained significant attention due to its involvement in the protein translocation pathway, which is essential for chloroplast biogenesis and functionality. Disruption of this pathway can lead to impaired photosynthesis and plant growth, making CIAO1 a valuable target for understanding chloroplast development and its regulation. Studies have identified that CIAO1 interacts with various precursor proteins, facilitating their recognition and import into chloroplasts. Moreover, the characterization of CIAO1 can provide insights into the evolutionary adaptations of plants to environmental stress, as chloroplasts are vital for energy production and metabolic processes. Additionally, investigations into CIAO1 may have broader implications for agricultural biotechnology, as enhancing chloroplast function could improve crop yield and stress resistance. Therefore, the study of CIAO1 not only enriches our understanding of plant biology but also paves the way for potential applications in sustainable agriculture.
