Analytical Data
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基因名
SLC46A1
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简介
The SLC46A1 protein is a proton-coupled folate symporter that utilizes the H(+) gradient in the proximal jejunum to drive folate absorption. It transports folate from the blood to the cerebrospinal fluid and exhibits alternating outward- and inward-open conformational states at acidic pH. SLC46A1 Protein, Human (sf9, His, MBP, FLAG) is the recombinant human-derived SLC46A1 protein, expressed by sf9 insect cells , with N-MBP, C-Flag, N-8*His labeled tag.
- Application
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别名
SLC46A1; Proton-coupled folate transporter; G21; Heme carrier protein 1; PCFT/HCP1; Solute carrier family 46 member 1
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种属
Human
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表达系统
Baculovirus
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标签
N-MBP;C-Flag;N-8*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96NT5
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表达区间
E2-P459
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蛋白长度
Partial
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SLC46A1, also known as the proton-coupled folate transporter (PCFT), plays a crucial role in the cellular uptake of folate and other related compounds. Folate is an essential vitamin that is vital for DNA synthesis, repair, and methylation, making SLC46A1 critical for cellular health and growth. Mutations in the SLC46A1 gene have been associated with various health conditions, such as hereditary folate malabsorption, leading to severe neurological disorders and anemia. Researchers have been focusing on the recombinant expression of SLC46A1 to better understand its structural and functional properties, as well as its transport mechanism. The production of recombinant SLC46A1 in heterologous systems allows for detailed studies on its pharmacological potential, interactions with substrates, and the impact of specific mutations. Understanding the transport kinetics and substrate specificity of SLC46A1 is essential not only for elucidating its physiological role but also for developing therapeutic strategies for diseases linked to folate dysregulation. As folate is a critical factor in cancer biology, exploring the functional dynamics of SLC46A1 may also provide insights into targeted therapies for malignancies. Overall, the research on SLC46A1 recombinant protein aims to bridge the gap between fundamental biology and potential clinical applications, paving the way for novel interventions for disorders associated with folate transport deficiencies.
