Analytical Data
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Gene name
SLC46A1
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简介
The SLC46A1 protein is a proton-coupled folate symporter that utilizes the H(+) gradient in the proximal jejunum to drive folate absorption. It transports folate from the blood to the cerebrospinal fluid and exhibits alternating outward- and inward-open conformational states at acidic pH. SLC46A1 Protein, Human (sf9, His, MBP, FLAG) is the recombinant human-derived SLC46A1 protein, expressed by sf9 insect cells , with N-MBP, C-Flag, N-8*His labeled tag.
- Application
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Alternative Names
SLC46A1; Proton-coupled folate transporter; G21; Heme carrier protein 1; PCFT/HCP1; Solute carrier family 46 member 1
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Species
Human
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Source
Baculovirus
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Tag
N-MBP;C-Flag;N-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96NT5
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Expression Region
E2-P459
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SLC46A1, also known as the proton-coupled folate transporter (PCFT), plays a crucial role in the cellular uptake of folate and other related compounds. Folate is an essential vitamin that is vital for DNA synthesis, repair, and methylation, making SLC46A1 critical for cellular health and growth. Mutations in the SLC46A1 gene have been associated with various health conditions, such as hereditary folate malabsorption, leading to severe neurological disorders and anemia. Researchers have been focusing on the recombinant expression of SLC46A1 to better understand its structural and functional properties, as well as its transport mechanism. The production of recombinant SLC46A1 in heterologous systems allows for detailed studies on its pharmacological potential, interactions with substrates, and the impact of specific mutations. Understanding the transport kinetics and substrate specificity of SLC46A1 is essential not only for elucidating its physiological role but also for developing therapeutic strategies for diseases linked to folate dysregulation. As folate is a critical factor in cancer biology, exploring the functional dynamics of SLC46A1 may also provide insights into targeted therapies for malignancies. Overall, the research on SLC46A1 recombinant protein aims to bridge the gap between fundamental biology and potential clinical applications, paving the way for novel interventions for disorders associated with folate transport deficiencies.
