Analytical Data
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Gene name
SLC21A3
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简介
SLC21A3 is a Na(+)-independent transporter that greatly facilitates cellular uptake of a variety of organic anions, including bile salts necessary for intestinal absorption. SLC21A3 is responsible for transporting substances such as DHEAS, estrone 3-sulfate, and atROL in human retinal pigment epithelial cells to maintain the integrity of the visual cycle. SLC21A3 Protein, Human (sf9, His, MBP, FLAG) is the recombinant human-derived SLC21A3 protein, expressed by sf9 insect cells , with N-MBP, C-Flag, N-8*His labeled tag.
- Application
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Alternative Names
SLCO1A2; Solute carrier organic anion transporter family member 1A2; OATP-A; Organic anion-transporting polypeptide 1; OATP-1; Sodium-independent organic anion transporter; Solute carrier family 21 member 3
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Species
Human
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Source
Baculovirus
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Tag
N-MBP;C-Flag;N-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P46721
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Expression Region
G2-L670
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SLC21A3, also known as Organic Anion Transporter 1 (OAT1), is a member of the solute carrier family that plays a crucial role in the renal excretion of various endogenous and xenobiotic compounds, including drugs, bilirubin, and metabolites. It is primarily expressed in the kidney, where it facilitates the uptake of organic anions from the bloodstream into renal tubular cells, significantly influencing drug pharmacokinetics and toxicity. Research on SLC21A3 is essential due to its implications in drug interactions, acute kidney injury, and renal diseases. Alterations in SLC21A3 expression and function can lead to impaired drug clearance and increased serum levels of potentially toxic substances. Therefore, understanding the molecular mechanisms governing SLC21A3 activity is vital for improving drug delivery systems and personalized medicine approaches. Further studies, particularly those involving the production and characterization of recombinant SLC21A3 proteins, are crucial for elucidating the structure-function relationships of this transporter and for screening potential inhibitors or substrates, contributing to better therapeutic strategies and the development of safer pharmaceuticals.
