Analytical Data
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基因名
SF3B3
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简介
SF3B3 protein is a key splicing factor in the SF3B complex and plays a central role in pre-mRNA splicing. It is essential for "A" complex assembly and stabilizes U2 snRNP binding to branch point sequences. SF3B3 Protein, Human (sf9) is the recombinant human-derived SF3B3 protein, expressed by sf9 insect cells , with tag free.
- Application
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别名
SF3B3; Splicing factor 3B subunit 3; Pre-mRNA-splicing factor SF3b 130 kDa subunit; SF3b130; STAF130; Spliceosome-associated protein 130; SAP 130
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种属
Human
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表达系统
Baculovirus
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标签
Tag Free
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q15393
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表达区间
F2-F1217
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蛋白长度
Partial
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SF3B3 is a crucial component of the spliceosome, the cellular machinery responsible for the splicing of pre-mRNA. As an essential part of the SF3B complex, SF3B3 plays a significant role in the regulation of gene expression, influencing the precise removal of introns and the joining of exons. Dysregulation of splicing, often linked to mutations or altered expression of splicing factors like SF3B3, has been implicated in various diseases, including cancer and neurodegenerative disorders. Recent studies have focused on the structural and functional characterization of SF3B3, revealing its involvement in the selection of splice sites and its interactions with other splicing factors. By using recombinant DNA technology, researchers can produce SF3B3 proteins for in vitro studies, allowing for a better understanding of its functional mechanisms. These studies are essential for elucidating the molecular pathways affected by SF3B3 and for developing potential therapeutic strategies targeting splicing dysregulation in various diseases. Overall, the investigation of SF3B3 recombinant proteins holds promise for contributing to the broader understanding of gene regulation and the splicing process in eukaryotic cells.
