Analytical Data
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Gene name
REG-3 alpha/REG3A
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简介
REG-3 α/REG3A protein is a bactericidal C-type lectin lacking an EPN motif that acts as a hormone in response to stimuli such as IL17A and the intestinal microbiome. It is secreted by various cell types and activates the receptor EXTL3, initiating cell-specific signaling pathways. REG-3 alpha/REG3A Protein, Rat (HEK293, Fc) is the recombinant rat-derived REG-3 alpha/REG3A protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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Alternative Names
Regenerating islet-derived protein 3-alpha; REG-3-alpha; Lithostathine 3; PAP2
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Species
Rat
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P35231
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Expression Region
E26-Q174
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Protein Length
Full Length of Mature Protein
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Molecular Weight
44 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
REG-3 alpha, also known as REG3A, is a member of the regenerating gene family and is predominantly expressed in the gastrointestinal tract, particularly in Paneth cells of the small intestine. Its discovery dates back to studies on the regenerative processes in the pancreas and has since garnered attention due to its antimicrobial properties and role in host defense mechanisms. REG3A is recognized for its ability to inhibit bacterial growth and modulate inflammation, making it a key player in maintaining intestinal homeostasis and a potential biomarker for various gastrointestinal disorders. Recent research has highlighted its involvement in conditions such as inflammatory bowel disease (IBD), pancreatitis, and colorectal cancer, where altered REG3A expression levels correlate with disease progression and severity. The recombinant protein form of REG3A is being explored not only for its therapeutic potential in enhancing mucosal healing and immune responses but also for its utility in understanding the molecular pathways of intestinal diseases. As such, REG3A has emerged as a significant target in studies aimed at unraveling the complexities of gut microbiota interactions and the immune response, prompting further investigation into its potential as a therapeutic agent.
