Recombinant Human GGH/Glutamyl hydrolase gamma Protein (HEK293)，His

GGH/Glutamyl hydrolase gamma

GGH (Glutamyl hydrolase gamma) protein hydrolyzes polyglutamate sidechains in pteroylpolyglutamates, progressively removing gamma-glutamyl residues to produce pteroyl-alpha-glutamate (folic acid) and free glutamate. Studies indicate GGH's crucial role in modulating the bioavailability of dietary pteroylpolyglutamates and its significance in the metabolism of both pteroylpolyglutamates and antifolates. GGH/Glutamyl hydrolase gamma Protein, Human (HEK293, His) is the recombinant human-derived GGH/Glutamyl hydrolase gamma protein, expressed by HEK293 , with C-6*His labeled tag.

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

Gamma-glutamyl hydrolase; Conjugase; GH; Gamma-Glu-X carboxypeptidase

Human

HEK293

C-6*His

Greater than 90% as determined by SDS-PAGE.

Q92820

M25-D318

RPHGDTAKKPIIGILMQKCRNKVMKNYGRYYIAASYVKYLESAGARVVPVRLDLTEKDYEILFKSINGILFPGGSVDLRRSDYAKVAKIFYNLSIQSFDDGDYFPVWGTCLGFEELSLLISGECLLTATDTVDVAMPLNFTGGQLHSRMFQNFPTELLLSLAVEPLTANFHKWSLSVKNFTMNEKLKKFFNVLTTNTDGKIEFISTMEGYKYPVYGVQWHPEKAPYEWKNLDGISHAPNAVKTAFYLAEFFVNEARKNNHHFKSESEEEKALIYQFSPIYTGNISSFQQCYIFD

Full Length of Mature Protein

40 kDa.

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.