Analytical Data
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Gene name
14-3-3 theta
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简介
14-3-3 theta proteins are adapters in multiple signaling pathways that recognize phosphoserine or phosphothreonine motifs and modulate chaperone activity upon binding. It negatively regulates PDPK1 kinase activity, forms homodimers, and interacts with CDK16, phosphorylated RGS7, SSH1, phosphorylated CDKN1B, GAB2, phosphorylated PDPK1, and phosphorylated DAPK2. 14-3-3 theta Protein, Human (GST) is the recombinant human-derived 14-3-3 theta protein, expressed by E. coli , with N-GST labeled tag.
- Application
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Alternative Names
14-3-3 protein theta; 14-3-3 protein tau; Protein HS1; YWHAQ
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Species
Human
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Source
E. coli
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Tag
N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P27348
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Expression Region
M1-N245
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Protein Length
Full Length
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Molecular Weight
53 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
14-3-3 theta is a member of the 14-3-3 protein family, which plays critical roles in various cellular processes, including cell growth, differentiation, and stress responses. Research into 14-3-3 theta has gained momentum due to its involvement in important signaling pathways, particularly those related to cancer progression and neurological disorders. These proteins function as molecular scaffolds, modulating the activity of numerous target proteins by binding to specific phosphoserine-containing motifs. The unique expression pattern of 14-3-3 theta in specific tissues and its differential regulation in pathological conditions underscore its potential as a biomarker and therapeutic target. Recent studies have indicated that alterations in the expression levels of 14-3-3 theta may influence cellular responses to stressors, suggesting its role in maintaining cellular homeostasis. Understanding the precise mechanisms by which 14-3-3 theta interacts with its partners could provide insights into its functional significance and contribute to the development of new strategies for treating diseases where these interactions are disrupted. This ongoing research aims to unravel the complex regulatory networks involving 14-3-3 theta, highlighting its significance in health and disease.
