Analytical Data
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Gene name
S100B
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简介
The S100B protein binds calcium and zinc ions, each with a different site on the monomer. Physiological levels of potassium ions disrupt the binding of calcium and zinc, affecting high-affinity sites. S100B Protein, Human (His) is the recombinant human-derived S100B protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Biological Activity
Measured in a cell proliferation assay using THP-1 cells. The ED50 for this effect is 0.09308 μg/mL, corresponding to a specific activity is 1.07×104 units/mg. Measured in a cell proliferation assay using THP-1 cells. The ED50 for this effect is 0.09308 μg/mL, corresponding to a specific activity is 1.07×104 units/mg.
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Alternative Names
Protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B; S100b; S100 beta; S100 calcium binding protein B
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P04271
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Expression Region
M1-E92
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Protein Length
Full Length
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Molecular Weight
12.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
S100B is a member of the S100 protein family, which consists of small, calcium-binding proteins that play crucial roles in various cellular processes, including cell cycle regulation, differentiation, and cellular signaling. S100B is predominantly expressed in astrocytes of the central nervous system (CNS) and is involved in neuroinflammatory responses and neuroprotection. Research has indicated that elevated levels of S100B in the serum or cerebrospinal fluid are associated with various neurological disorders, such as traumatic brain injury, Alzheimer's disease, and multiple sclerosis, making it a potential biomarker for neurological conditions. The recombinant production of S100B protein allows for detailed biochemical studies, including structural analysis and functional assays, which can reveal insights into its role in neurobiology and its pathological implications in brain disorders. Additionally, recombinant S100B can be used to explore therapeutic avenues, such as drug discovery and development, as well as in creating targeted treatments for diseases associated with altered S100B levels. Overall, the study of recombinant S100B protein continues to be a critical area of research in understanding brain function and the molecular mechanisms underlying various CNS pathologies.
