Analytical Data
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基因名
MeCP2
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简介
The MeCP2 protein is a chromosome binder that specifically binds to methylated DNA independent of flanking sequences. MeCP2 Protein, Human (HEK293, His) is the recombinant human-derived MeCP2 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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别名
rHuMethyl-CpG-binding protein 2/MECP2, His; Methyl-CpG-binding protein 2; MECP2; MeCp-2 protein
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P51608-1
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表达区间
M1-S486
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氨基酸序列
MVAGMLGLREEKSEDQDLQGLKDKPLKFKKVKKDKKEEKEGKHEPVQPSAHHSAEPAEAGKAETSEGSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS
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蛋白长度
Full Length of Isoform-1
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分子量
83.0-90.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MeCP2 (Methyl-CpG-binding protein 2) is a key regulatory protein that binds to methylated DNA and is crucial for neuronal development and maintenance. Mutations in the MECP2 gene are associated with Rett syndrome, a neurodevelopmental disorder primarily affecting females, leading to severe cognitive and physical impairments. While MeCP2 is essential for normal brain function, its overexpression or dysregulation can lead to various neuropsychiatric conditions. The study of recombinant MeCP2 protein facilitates understanding its structural and functional properties, enabling researchers to dissect the molecular mechanisms underlying its role in gene regulation and cellular processes. Recombinant MeCP2 is produced in laboratory settings to investigate its interaction with DNA and other proteins, providing insights into its function in chromatin remodeling and transcriptional regulation. Moreover, developing therapeutic strategies targeting MeCP2-related pathways holds promise for treating Rett syndrome and other related disorders. By exploring the nuances of MeCP2's functionality through recombinant approaches, researchers aim to uncover potential interventions that can restore normal neural function in affected individuals, offering hope for future therapeutic advancements.
