Recombinant Mouse SMPD1 Protein (Baculovirus)，His

SMPD1

The SMPD1 protein converts sphingomyelin to ceramide through two enzymatic forms, one targeted to endolysosomes and the other released extracellularly.Lysosomal exocytosis becomes a significant source of the secretory form during stress responses.SMPD1 also exports cholesterol from intraendolysosomal membranes and exhibits phospholipase C activities.Its role in stress-induced apoptosis modulation is mediated through ceramide production.SMPD1 Protein, Mouse (sf9, His) is the recombinant mouse-derived SMPD1 protein, expressed by Sf9 insect cells , with C-His labeled tag.

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

Sphingomyelin phosphodiesterase; Acid sphingomyelinase; ASMase; Smpd1; Asm

Mouse

Baculovirus

C-His

Greater than 90% as determined by SDS-PAGE.

L45-L626

Partial

63 kDa.

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.