Analytical Data
-
Gene name
PTPRC/CD45RA
-
简介
Receptor-type tyrosine-protein phosphatase C (PTPRC) is a receptor type PTP and an essential positive regulator of T- and B-cell antigen receptor signaling. PTPRC functions through direct interaction with antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. PTPRC also suppresses JAK kinases as a regulator of cytokine receptor signaling. PTPRC/CD45RA Protein, Human (HEK293, His) is the recombinant human-derived PTPRC/CD45RA protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
-
Alternative Names
rHuReceptor-type tyrosine-protein phosphatase C/CD45RA, His ; B220; CD45 antigen; CD45; CD45R; LCA; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; PTPRC; receptor-type tyrosine-protein phosphatase C
-
Species
Human
-
Source
HEK293
-
Tag
C-6*His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P08575-8
-
Expression Region
Q26-K482
-
AA Sequence
QSPTPSPTGLTTAKMPSVPLSSDPLPTHTTAFSPASTFERENDFSETTTSLSPDNTSTQVSPDSLDNASAFNTTDAYLNASETTTLSPSGSAVISTTTIATTPSKPTCDEKYANITVDYLYNKETKLFTAKLNVNENVECGNNTCTNNEVHNLTECKNASVSISHNSCTAPDKTLILDVPPGVEKFQLHDCTQVEKADTTICLKWKNIETFTCDTQNITYRFQCGNMIFDNKEIKLENLEPEHEYKCDSEILYNNHKFTNASKIIKTDFGSPGEPQIIFCRSEAAHQGVITWNPPQRSFHNFTLCYIKETEKDCLNLDKNLIKYDLQNLKPYTKYVLSLHAYIIAKVQRNGSAAMCHFTTKSAPPSQVWNMTVSMTSDNSMHVKCRPPRDRNGPHERYHLEVEAGNTLVRNESHKNCDFRVKDLQYSTDYTFKAYFHNGDYPGEPFILHHSTSYNSK
-
Protein Length
Partial
-
Molecular Weight
120-150 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PTPRC, commonly known as CD45, is a critical receptor-type protein tyrosine phosphatase primarily expressed on the surface of immune cells, including T and B lymphocytes. It plays a pivotal role in regulating immune cell activation, differentiation, and signaling pathways. The isoform CD45RA is particularly significant, as it is associated with naive T cells and has been implicated in various immune responses and diseases. Research into the recombinant production of PTPRC/CD45RA protein allows scientists to obtain large quantities of this important molecule for various applications, including structural studies, functional assays, and therapeutic development. Understanding the functional implications of CD45RA on lymphocyte signaling can provide insights into the mechanisms underlying immune responses and the pathogenesis of disorders like autoimmunity and cancer. Moreover, recombinant CD45RA has potential as a biomarker for assessing T cell status and as a target for immunotherapy approaches. By producing and characterizing this protein, researchers aim to better understand its role in immune modulation and develop novel strategies to manipulate immune responses in clinical settings.
