Analytical Data
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Gene name
Grb2
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简介
Grb2, an adapter protein, crucially connects cell surface growth factor receptors to the Ras signaling pathway. Despite no direct binding to phosphorylated EGFR, Grb2 inhibits EGF-induced transactivation of a RAS-responsive element. It acts as a dominant negative relative to GRB2, suppressing proliferative signals and potentially initiating programmed cell death. Grb2 Protein, Human (His) is the recombinant human-derived Grb2 protein, expressed by E. coli , with C-6*His labeled tag.
- Application
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Biological Activity
1.Immobilized Human GRB2 at 2 μg/mL (100 μL/well) can bind Anti-GRB2 Antibody. The ED50 for this effect is 93.39 ng/mL. 2.Measured in a cell proliferation assay using A549 cells. The ED50 for this effect is ≤33.3 ng/mL, corresponding to a specific activity is 3.003×104 units/mg Measured in a cell proliferation assay using A549 cells. The ED50 for this effect is 33.3 ng/mL, corresponding to a specific activity is 3.003×104 units/mg.
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Alternative Names
rHuGrowth factor receptor-bound protein 2/GRB2, His; Growth Factor Receptor-Bound Protein 2; Adapter Protein GRB2; Protein Ash; SH2/SH3 Adapter GRB2; GRB2; ASH
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Species
Human
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Source
E. coli
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Tag
C-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P62993-1
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Expression Region
M1-V217
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AA Sequence
MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV
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Protein Length
Full Length of Isoform-1
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Molecular Weight
25-29 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Grb2 (Growth factor receptor-bound protein 2) is a key adaptor protein involved in various cellular signaling pathways, particularly those associated with growth factor receptor activation. Its primary role is to mediate signal transduction from receptor tyrosine kinases (RTKs) to downstream effectors, including the Ras-MAPK pathway, which is crucial for cellular processes such as proliferation, differentiation, and survival. Research into Grb2 recombinant protein has gained significant attention due to its central role in oncogenesis and various diseases, including cancer. The ability to produce Grb2 as a recombinant protein allows for detailed biochemical characterization, including studies on its binding affinities and interaction with other signaling molecules. Additionally, understanding the structural dynamics of Grb2 through methods such as X-ray crystallography or NMR spectroscopy can provide insights into its functional mechanisms. Furthermore, the development of Grb2 inhibitors or modulators holds therapeutic potential, particularly in targeting cancers that exhibit aberrant RTK signaling. Thus, the study of Grb2 recombinant protein is essential for elucidating its role in cellular signaling and for advancing potential therapeutic strategies aimed at diseases related to dysregulated signaling pathways. This research can ultimately contribute to the identification of novel drug targets, enhancing our understanding of cellular communications and pathophysiological conditions associated with Grb2 activity.
