Analytical Data
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Gene name
HHLA2
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简介
HHLA2 protein, a key immune regulator, interacts with TMIGD2, providing crucial costimulation for T-cells during TCR-mediated activation. This interaction amplifies T-cell proliferation and cytokine production through an AKT-dependent signaling cascade, revealing HHLA2's role in enhancing T-cell responses and immune modulation. HHLA2 Protein, Human (HEK293, Fc) is the recombinant human-derived HHLA2 protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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Biological Activity
1.Measured by its binding ability in a functional ELISA. 2.Immobilized human TMIGD2 at 10 μg/mL (100 μL/well) can bind human HHLA2-Fc, the EC50 of human HHLA2-Fc is 0.3-3 μg/mL. 3.Loaded Recombinant Human KIR3DL3 Protein, His Tag on His1K Biosensor, can bind Recombinant Human B7-H7/HHLA2 Protein, hFc Tag with an affinity constant of 51.8 nM as determined in BLI assay (Sartorius Octet RED384)
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Alternative Names
HERV-H LTR-associating protein 2; Human endogenous retrovirus-H long terminal repeat-associating protein 2
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Species
Human
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UM44
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Expression Region
I23-N344
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Protein Length
Partial
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Molecular Weight
63.7 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HHLA2, or human endogenous retrovirus-H long terminal repeat-associated protein 2, belongs to the B7 family of immune regulatory molecules. Its expression is mainly observed in immune cells, particularly in dendritic cells and macrophages. Given its role in modulating immune responses, HHLA2 has attracted significant attention in cancer immunotherapy research. Studies have indicated that HHLA2 can inhibit T-cell activation and function, suggesting it may act as a negative regulator of the immune system. This immunosuppressive property raises questions about its potential involvement in tumor evasion mechanisms. Consequently, researchers are investigating HHLA2 as a target for cancer treatment, aiming to block its function and enhance the anti-tumor immune response. The development of recombinant HHLA2 proteins serves as a valuable tool for elucidating the precise mechanisms by which this protein influences immune cells and for exploring its implications in various clinical settings, including cancer therapy. Understanding the biological functions and pathways associated with HHLA2 could pave the way for novel therapeutic strategies that leverage immune modulation to improve patient outcomes in cancer and other diseases characterized by immune dysregulation.
