Analytical Data
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Gene name
Transferrin
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简介
Transferrin is an important iron-binding transport protein that binds two Fe(3+) ions, usually with anions such as bicarbonate. Crucially, it transports iron from the site of absorption and heme degradation to the site of storage and utilization. Transferrin Protein, Pig (HEK293, His) is the recombinant pig-derived Transferrin protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Biological Activity
Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is 22.35 ng/mL, corresponding to a specific activity is 4.47×104 units/mg. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is 22.35 ng/mL, corresponding to a specific activity is 4.47×104 units/mg.
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Alternative Names
Serotransferrin; Transferrin; Beta-1 metal-binding globulin; Siderophilin; TF; TRF
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Species
Pig
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Source
HEK293
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Tag
C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
B3CL06
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Expression Region
Q22-T715
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AA Sequence
QKTVRWCTISNQEANKCSSFRENMSKAVKNGPLVSCVKKSSYLDCIKAIRDKEADAVTLDAGLVFEAGLAPYNLKPVVAEFYGQKDNPQTHYYAVAVVKKGSNFQWNQLQGKRSCHTGLGRSAGWIIPMGLLYDQLPEPRKPIEKAVASFFSSSCVPCADPVNFPKLCQQCAGKGAEKCACSNHEPYFGYAGAFNCLKEDAGDVAFVKHSTVLENLPDKADRDQYELLCRDNTRRPVDDYENCYLAQVPSHAVVARSVDGQEDSIWELLNQAQEHFGRDKSPDFQLFSSSHGKDLLFKDSANGFLRIPSKMDSSLYLGYQYVTALRNLREEISPDSSKNECKKVRWCAIGHEETQKCDAWSINSGGKIECVSAENTEDCIAKIVKGEADAMSLDGGYIYIAGKCGLVPVLAENYKTEGENCVNTPEKGYLAVAVVKKSSGPDLNWNNLKGKKSCHTAVDRTAGWNIPMGLLYNKINSCKFDQFFGEGCAPGSQRNSSLCALCIGSERAPGRECLANNHERYYGYTGAFRCLVEKGDVAFVKDQVVQQNTDGKNKDDWAKDLKQMDFELLCQNGAREPVDNAENCHLARAPNHAVVARDDKVTCVAEELLKQQAQFGRHVTDCSSSFCMFKSNTKDLLFRDDTQCLARVGKTTYESYLGADYITAVANLRKCSTSKLLEACTFHSAKNPRVETTT
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Protein Length
Partial
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Molecular Weight
80 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Transferrin is a key glycoprotein involved in iron transport and homeostasis in the human body. It plays a critical role in cellular iron uptake by binding ferric iron (Fe^3+) and facilitating its delivery to various tissues through receptor-mediated endocytosis. The study of recombinant transferrin has gained significant attention in recent years due to its potential applications in medicine and biotechnology. Recombinant technology allows for the production of modified versions of transferrin that may enhance its iron-binding capacity, stability, and specificity. These engineered proteins can be explored for therapeutic uses, such as in the treatment of iron deficiency anemia or as carriers for targeted drug delivery—leveraging the natural uptake mechanisms of transferrin to target specific cells, thereby improving treatment efficacy and reducing side effects. Furthermore, understanding the structure-function relationship of transferrin through recombinant studies can provide insights into the mechanisms of iron metabolism and its dysregulation in various diseases, including cancer and neurodegenerative disorders. Overall, the research on recombinant transferrin not only contributes to our fundamental understanding of iron biology but also holds promise for innovative therapeutic strategies.
