Analytical Data
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Gene name
LSM4
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简介
The LSM4 protein plays a crucial role in pre-mRNA splicing, participating in the U4/U6-U5 tri-snRNP complex during spliceosome assembly, and as a component of the precatalytic spliceosome (spliceosome B complex).In the heptameric LSM2-8 complex, LSM4 specifically binds to the 3' U region of U6 snRNA.LSM4 Protein, Human (His) is the recombinant human-derived LSM4 protein, expressed by E.coli , with N-6*His labeled tag.
- Application
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Alternative Names
U6 snRNA-Associated Sm-Like Protein LSm4; Glycine-Rich Protein; GRP; LSM4
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y4Z0
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Expression Region
M1-Q139
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Protein Length
Full Length
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Molecular Weight
17 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The LSM4 (Like Sm protein 4) protein plays a crucial role in the regulation of RNA metabolism and processing, particularly in the context of mRNA stability and splicing. It is part of the LSM (Like Sm) protein family, which is characterized by their shared structure and function in RNA binding. Research into LSM4 has gained traction due to its involvement in various cellular processes, including the formation of ribonucleoprotein complexes and participation in the decay of defective mRNA. Studies have shown that LSM4 interacts with several key proteins and RNA molecules, highlighting its importance in gene expression regulation. Additionally, the dysregulation of LSM4 has been implicated in various diseases, including cancer, making it a potential target for therapeutic intervention. Recent advances in recombinant protein technology have enabled researchers to produce LSM4 in vitro, allowing for detailed structural and functional studies. This research not only enhances our understanding of LSM4's biological roles but also paves the way for exploring its potential applications in biotechnology and medicine, such as the development of RNA-based therapies. Thus, the investigation of LSM4 recombinant proteins is a promising frontier in molecular biology, with implications spanning from fundamental research to clinical applications.
