Analytical Data
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Gene name
CLEC-2
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简介
The CLEC1B/CLEC-2 protein serves as a platelet receptor for PDPN. When activated, it signals through SRC and SYK kinases, activating PLCG2. CLEC1B forms homodimers and interacts with RACK1, promoting its ubiquitination and degradation. It also interacts with SYK and PDPN, independently of glycosylation, activating CLEC1B/CLEC-2. CLEC1B/CLEC-2 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived CLEC1B/CLEC-2 protein, expressed by HEK293 , with N-hFc labeled tag.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Podoplanin is coated at 1.0 µg/mL (100 μL/well), Recombinant Mouse CLEC-2 binds with ED50 of 0.2387 μg/mL. Measured by its binding ability in a functional ELISA. When Recombinant Mouse Podoplanin is coated at 1.0 µg/mL (100 μL/well), Recombinant Mouse CLEC-2 binds with ED50 of 0.2387 μg/mL.
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Alternative Names
C-type lectin domain family 1 member B; CLEC1B; CLEC2
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Species
Mouse
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Source
HEK293
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Tag
N-hFc
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q9JL99-1
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Expression Region
M53-L229
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Protein Length
Extracellular Domain
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Molecular Weight
50-65 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CLEC-2 (C-type lectin-like receptor 2) is a pivotal receptor primarily expressed on dendritic cells and platelets, playing a crucial role in the immune system and hemostasis. It is known for its ability to recognize specific glycans found on pathogens and activated platelets, thereby mediating immune responses and thrombosis. The significance of CLEC-2 in various physiological and pathological processes has garnered considerable attention, particularly in tumor immunity and infectious diseases. Research indicates that CLEC-2 is involved in dendritic cell maturation and T-cell activation, influencing how the immune system responds to foreign antigens. Additionally, its role in platelet activation suggests that CLEC-2 could be a potential therapeutic target for anticoagulant strategies. Given the increasing incidence of thromboembolic diseases globally, understanding CLEC-2's structure and function is critical for developing novel treatments. Consequently, recombinant CLEC-2 protein research has become a focal point, aiming to elucidate its molecular mechanisms, interactions, and potential applications in immunotherapy and thrombosis management. Moreover, producing recombinant CLEC-2 enables the exploration of its structural biology and can facilitate the design of specific inhibitors or agonists to modulate its activity in clinical settings. The investigation of CLEC-2 continues to be a promising area of research in immunology and hematology, with implications for enhancing therapeutic strategies against various diseases.
