Analytical Data
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Gene name
CD40L/CD154/TRAP
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简介
CD40L (CD154; TRAP) is a ligand to CD40/TNFRSF5, acts function by generating a costimulatory signal that up-regulates IL-4 synthesis[1]. CD40L is specifically expressed on activated CD4+ T-lymphocytes and involves in activation of NF-κB/MAPK pathway[2][3]. CD40L also involves in B cell differentiation, maturation, and apoptosis[4]. CD40L in human, cleaved into 2 chains of membrane form (1-261 a.a.) and soluble form (113-261 a.a.) which serves as a ligand for integrins (ITGA5:ITGB1 and ITGAV:ITGB3). CD40L/CD154/TRAP Protein, Human has a total length of 149 amino acids (M113-L261), is expressed in E. coli cells with tag free.
- Application
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Biological Activity
1. Measured in a cell proliferation assay using human B cells (Ramos) in the presence of 10 ng/mL Human IL-4. The ED50 this effect is 1-3 ng/mL. 2.The ED50 as determined by the dose-dependant stimulation of IL-12 & IL-8 induction by PMB (Peripheral Mononuclear) cells was found to be <20 μg/mL. Measured by its binding ability in a functional ELISA. Immobilized Mouse TREM2, at 1 μg/mL (100 μL/well) can bind Anti-TREM2 Antibody, the ED50 is 46.44 ng/mL, corresponding to a specific activity is 2.153×104 units/mg.
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Alternative Names
rHuCD40L; CD154; TRAP; TNFSF5; CD40LG; CD40-L
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P29965
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Expression Region
M113-L261
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Protein Length
Full Length of CD40L soluble form
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Molecular Weight
16.44 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD40L, also known as CD154, is a crucial membrane protein primarily expressed on activated T cells and plays a pivotal role in immune system regulation by providing essential signals to B cells and other antigen-presenting cells through CD40 receptor engagement. This interaction is vital for processes such as B cell activation, proliferation, and isotype switching, all of which are fundamental for generating effective adaptive immune responses. In addition, CD40L is implicated in various immunological disorders, including autoimmune diseases and cancers, making it an attractive target for therapeutic interventions. To study its functions and potential applications, researchers have developed recombinant CD40L proteins, often referred to as TRAP (trimeric association protein), which can mimic the natural signaling of CD40L. These recombinant proteins facilitate the investigation of CD40/CD40L pathways in controlled settings, enabling the exploration of their roles in immune responses and paving the way for innovative treatments that harness this pathway. Understanding the structure and function of CD40L/TRAP enhances our knowledge of immune mechanisms and could lead to novel strategies for modulating immune responses in various diseases.
