Analytical Data
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Gene name
IL-7
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简介
IL-7 protein regulates lymphocyte population and maintains lymphoid homeostasis by developing, expanding, and supporting survival of T-cells and B-cells. It interacts with IL7R and CSF2RG receptors to exert its effects. IL-7 Protein, Mouse is the recombinant mouse-derived IL-7 protein, expressed by E. coli , with tag free.
- Application
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Biological Activity
1.Measured in a cell proliferation assay using antibody against CD3-activated human peripheral blood lymphocytes (PBL) and the ED50 is typically 0.4-5.2 ng/mL. 2.Measured in a cell proliferation assay using antibody against CD3-activated human peripheral blood mononuclear cell (PBMC). The ED50 for this effect is typically 0.4-4 ng/mL. 3.Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL-7 is immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated Recombinant Human IL-7 R alpha. The ED50 for this effect is 1.814 ng/mL. Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL-7 is immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated Recombinant Human IL-7 R alpha. The ED50 for this effect is 1.814 ng/mL.
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Alternative Names
Interleukin 7; Lymphopoietin-1; PBGF
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Species
Mouse
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by reducing SDS-PAGE. as determined by SDS-PAGE.
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Uniprot
P10168/Q544C8
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Expression Region
E26-I154
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Protein Length
Full Length of Mature Protein
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Molecular Weight
18 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
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Protein Description
Interleukin-7 (IL-7) is a crucial cytokine involved in the development, survival, and maintenance of T and B lymphocytes, playing a vital role in the immune system. Research on recombinant IL-7 protein has gained significant attention due to its potential therapeutic applications in immunodeficiency disorders, such as HIV/AIDS, and in enhancing anti-tumor immunity in cancer treatments. In the context of HIV, IL-7 can stimulate the proliferation of T-cells, potentially restoring immune function in infected individuals. Moreover, studies suggest that IL-7 may aid in expanding T-cell populations, which is essential for effective vaccine responses and for improving overall immune surveillance against tumors. The recombinant form of IL-7 can be produced using various expression systems, allowing for large-scale production and facilitating its use in clinical settings. Additionally, ongoing research is investigating the safety, dosage, and efficacy of IL-7 in combination therapies for both immunotherapy and regenerative medicine. Understanding the mechanisms by which IL-7 regulates lymphocyte homeostasis and function will not only enhance our knowledge of immune system dynamics but also pave the way for innovative strategies to harness its properties for therapeutic benefit.
