Analytical Data
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Gene name
PDGF R alpha
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简介
PDGF R beta, a tyrosine-protein kinase receptor, crucially regulates embryonic development, overseeing cell processes like proliferation, survival, differentiation, chemotaxis, and migration. Especially vital in blood vessel development, it facilitates pericyte and smooth muscle cell proliferation, migration, and recruitment to endothelial cells, playing a significant role in blood vessel formation and maintenance. PDGF R beta Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived PDGF R beta protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Biological Activity
Measured by its ability to inhibit the biological activity of PDGF-BB using NIH-3T3 mouse fibroblast cells. The ED50 for this effect is 2.236 µg/mL in the presence of 50 ng/mL Recombinant Human PDGF-BB, corresponding to a specific activity is 447.22 Unit/mg. Measured by its ability to inhibit the biological activity of PDGF-BB using NIH-3T3 mouse fibroblast cells. The ED50 for this effect is 2.236 µg/mL in the presence of 50 ng/mL Recombinant Human PDGF-BB, corresponding to a specific activity is 447.22 unit/mg.
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Alternative Names
Platelet-derived growth factor receptor beta; PDGF-R-beta; PDGFR-1; CD140b; PDGFRB
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Species
Rhesus Macaque
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Source
HEK293
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Tag
C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
F7BTH1
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Expression Region
L33-F530
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AA Sequence
LVITPPGPELILNVSSTFVLTCSGSAPVVWERMSQELPQEMAKAQDNTFSSVLTLTNLTGLDTGEYFCTYNDSRGLEPDERKRLYIFVPDPTMGFLPNDAEELFIFLTEITEITIPCRVTDPQLVVTLHEKKGDIALPVPYDHQRGFSGIFEDRSYICKTTIGDREVDSDAYYVYRLQVSSINVSVNAVQTVVRQGENITLMCIVIGNEVVNFEWMYPRKESGRLVEPVTDFLLDMPYHIRSILHIPSAELEDSGTYTCNVTESVNDHQDEKAINITVVESGYVRLLGEVGALQFAELHRSRTLQVVFEAYPPPTVLWFKDNRTLGDSSAGEIALSTRNVSETRYVSELTLVRVKVAEAGHYTMRAFHEDAEVQLSFQLQINVPVRVLELSESHPDSGEQTVRCRGRGMPQPNIIWSACRDLKRCPRELPPMLLGNSSEEESQLETNVTYWEEEQEFEVVSTLRLQHVDRPLSVRCTLRNAVGQDMQEVIVVPHSLPF
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Protein Length
Partial
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Molecular Weight
80-96 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Platelet-derived growth factor receptor alpha (PDGF Rα) plays a crucial role in various cellular processes, including proliferation, migration, and survival, making it significant in developmental biology and tissue repair. It is primarily involved in mediating the effects of PDGF, a potent mitogen that regulates cell growth and angiogenesis. Research into PDGF Rα has gained momentum due to its implication in several pathologies, particularly in fibrotic diseases, atherosclerosis, and cancers, where abnormal PDGF signaling contributes to disease progression. The production of recombinant PDGF Rα proteins allows for detailed studies of its structure, function, and interactions at a molecular level. These recombinant proteins are essential for elucidating the receptor's role in signaling pathways and understanding how its dysregulation can lead to oncogenesis and other disorders. Additionally, the use of PDGF Rα as a therapeutic target is under investigation, as inhibiting its signaling may provide new avenues for treating diseases linked to abnormal cell proliferation and migration. Overall, the research on recombinant PDGF Rα not only enhances our understanding of fundamental biological processes but also opens up potential therapeutic strategies aimed at modulating its activity in various diseases.
