Analytical Data
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Gene name
BAFF/TNFSF13B
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简介
The BAFF/TNFSF13B protein is part of the TNF family and is critical in the regulation of immune responses and cell survival. It is also called B cell activating factor and plays a crucial role in B cell development, activation and survival by binding to receptors such as BAFF-R. BAFF/TNFSF13B Protein, Canine (HEK293, Fc) is the recombinant canine-derived BAFF/TNFSF13B protein, expressed by HEK293 , with N-hFc labeled tag. The total length of BAFF/TNFSF13B Protein, Canine (HEK293, Fc) is 150 a.a., with molecular weight of ~45.5 kDa.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. When Recombinant BCMA Protein is immobilized at 2 µg/mL (100 µL/well) can bind Recombinant Canine BAFF. The ED50 for this effect is 4.397 ng/mL. Measured by its binding ability in a functional ELISA. When Recombinant BCMA Protein is immobilized at 2 µg/mL (100 µL/well) can bind Recombinant Canine BAFF. The ED50 for this effect is 4.397 ng/mL.
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Alternative Names
Tumor necrosis factor ligand superfamily member 13B; BAFF; TALL-1
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Species
Canine
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Source
HEK293
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Tag
N-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
C4NZX1
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Expression Region
A143-L292
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Protein Length
Partial
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Molecular Weight
45-50 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BAFF (B-cell activating factor), also known as TNFSF13B, is a critical cytokine belonging to the tumor necrosis factor (TNF) superfamily that plays a vital role in B-cell development, activation, and survival. Dysregulation of BAFF levels has been implicated in various autoimmune diseases, such as systemic lupus erythematosus (SLE) and rheumatoid arthritis, as well as in certain B-cell malignancies. Understanding the structure and function of BAFF is essential for developing targeted therapies aimed at modulating immune responses. Research on recombinant BAFF proteins has gained momentum as these proteins can facilitate the investigation of BAFF's biological activities and mechanisms of action. Additionally, recombinant BAFF can be used as a tool for in vitro assays to explore B-cell signaling pathways and as a potential therapeutic agent to enhance or inhibit B-cell responses in various clinical settings. The generation of well-characterized recombinant BAFF proteins is pivotal for elucidating the cytokine's role in the immune system and for advancing novel treatments for B-cell-related disorders. Recent studies have focused on the production, purification, and functional characterization of these proteins, paving the way for better understanding of their implications in health and disease.
