Analytical Data
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基因名
CD117/c-Kit
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简介
CD117/c-kit is a tyrosine protein kinase receptor for KITLG/SCF that coordinates cell survival, proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration, and melanogenesis. Upon KITLG/SCF binding, CD117 activates the pathway and phosphorylates PIK3R1, PLCG1, SH2B2/APS, and CBL. CD117/c-kit Protein, Human (P10721-2, HEK293, Fc) is the recombinant human-derived CD117/c-kit protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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别名
Mast/stem cell growth factor receptor Kit; SCFR; CD117; Kit; Sl
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种属
Human
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表达系统
HEK293
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标签
C-hFc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P10721-2
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表达区间
Q26-T516
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蛋白长度
Partial
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分子量
93-115 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD117, also known as c-Kit, is a receptor tyrosine kinase that plays a crucial role in various biological processes, including cell survival, proliferation, and differentiation. It is predominantly expressed in hematopoietic stem cells, mast cells, and interstitial cells of Cajal, making it vital for normal physiological functions such as hematopoiesis and gastrointestinal motility. The study of CD117/c-Kit has significant implications in cancer research, particularly in understanding its mutation-driven malignancies like gastrointestinal stromal tumors (GISTs), where activating mutations lead to uncontrolled cell growth. Moreover, c-Kit is implicated in other cancer types, including melanoma and certain leukemias, highlighting its potential as a therapeutic target. Researchers have been focusing on developing recombinant proteins for c-Kit to elucidate its signaling pathways and investigate novel therapeutic strategies. These recombinant proteins can be utilized in assays to study interactions with downstream signaling molecules, assess the efficacy of small molecule inhibitors, and screen for c-Kit activating antibodies. Furthermore, elucidating the structure-function relationship of c-Kit through recombinant protein studies can aid in the design of more selective therapeutics aimed at targeting aberrant c-Kit signaling in cancer. Overall, the exploration of CD117/c-Kit recombinant proteins serves as a critical research avenue for advancing the understanding of its biological functions and developing targeted therapies for diseases associated with c-Kit dysregulation.
