Recombinant Human CTLA-4 Protein(HEK293), mFc

CTLA-4

The CTLA-4 protein is a key inhibitory receptor and a major negative regulator of T cell responses in coordination of immune regulation. Its unique property is its significantly increased affinity for B7 ligands (CD80/CD86) compared with CD28. CTLA-4 Protein, Human (HEK293, mFc) is the recombinant human-derived CTLA-4 protein, expressed by HEK293, with C-mFc labeled tag.

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

rHuCytotoxic T-lymphocyte protein 4/CTLA-4, Fc; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CTLA4; CD152

Human

HEK293

C-mFc

Greater than 90% as determined by SDS-PAGE.

P16410

A37-F162

50 kDa,based on SDS-PAGE under reducing conditions,100 kDa,based on SDS-PAGE under non-reduced conditions,due to the glycosylation.

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.