Analytical Data
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Gene name
Neprilysin/CD10
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简介
MME is a cell-surface peptidase member of the M13 family of zinc peptidases, which also includes endothelin converting enzymes (ECE-1 and ECE-2), KELL and PEX. MME cleaves peptide bonds on the amino side of hydrophobic amino acids and is the key enzyme in processing of a variety of physiologically active peptides, such as GRP, neurotensin (NT), and vasoactive intestinal peptide (VIP). MME is downregulated in nearly 50% of primary and metastatic prostate cancers. Neprilysin/CD10 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived Neprilysin/CD10 protein, expressed by HEK293 , with N-His labeled tag.
- Application
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Alternative Names
Neprilysin; Atriopeptidase; CALLA; NEP; SFE; CD10; MME
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Species
Rhesus Macaque
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Source
HEK293
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
F7H3Y6
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Expression Region
Y52-W750
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AA Sequence
YDDGICKSSDCIKSAARLIQNMDATAEPCADFFKYACGGWLKRNVIPETSSRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYNKMTLAQLQSNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKCRVW
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Protein Length
Partial
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Molecular Weight
95 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Neprilysin, also known as neutral endopeptidase or CD10, is an important zinc-dependent metallopeptidase involved in the degradation of bioactive peptides, including neuropeptides and natriuretic peptides. Its regulatory role in neuropeptide metabolism has garnered attention for its implications in various physiological and pathological processes, such as cardiovascular health, neurodegenerative diseases, and cancer. Neprilysin's activity contributes to the modulation of signaling pathways in the nervous system and plays a crucial role in maintaining blood pressure homeostasis. In cancer research, aberrant expression of neprilysin/CD10 has been linked to tumor progression and metastasis, making it a potential biomarker and therapeutic target. Understanding the structure, function, and regulation of neprilysin/CD10 through recombinant protein studies can provide insights into its biological roles and therapeutic potential. Recent advances in recombinant DNA technology have facilitated the production of high-purity neprilysin proteins, allowing for detailed biochemical characterization and the exploration of its interactions with peptide substrates. This research is pivotal for developing neprilysin inhibitors or enhancers that could be harnessed in treating diseases where neprilysin activity is compromised or dysregulated.
