Analytical Data
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基因名
ICAM-2/CD102
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简介
ICAM-2/CD102 protein belongs to the ICAM family of the immunoglobulin superfamily and may be involved in immune responses and cell interactions. This is consistent with the family's established role in immune processes. ICAM-2/CD102 Protein, Human (HEK293, His-Fc) is the recombinant human-derived ICAM-2/CD102 protein, expressed by HEK293 , with C-hFc, C-His labeled tag.
- Application
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生物活性
Determined by its ability to support the adhesion of PMA-stimulated jurkat cells to plates pre-coated with ICAM-2. The ED50 for this effect is 1.348 μg/mL, corresponding to a specific activity is 7.42×10^2 units/mg. Determined by its ability to support the adhesion of PMA-stimulated jurkat cells to plates pre-coated with ICAM-2. The ED50 for this effect is 1.348 μg/ml, corresponding to a specific activity is 7.42×102 units/mg.
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别名
Intercellular adhesion molecule 2; ICAM-2; CD102
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种属
Human
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表达系统
HEK293
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标签
C-hFc;C-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q6FHE2
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表达区间
K25-Q223
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氨基酸序列
KVFEVHVRPKKLAVEPKGSLEVNCSTTCNQPEVGGLETSLDKILLDEQAQWKHYLVSNISHDTVLQCHFTCSGKQESMNSNVSVYQPPRQVILTLQPTLVAVGKSFTIECRVPTVEPLDSLTLFLFRGNETLHYETFGKAAPAPQEATATFNSTADREDGHRNFSCLAVLDLMSRGGNIFHKHSAPKMLEIYEPVSDSQ
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蛋白长度
Partial
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分子量
85-95 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ICAM-2 (Intercellular Adhesion Molecule 2) is a member of the immunoglobulin superfamily and plays a crucial role in cell adhesion and immune responses. It primarily interacts with the leukocyte integrin CD102 (also known as LFA-1), facilitating the binding of leukocytes to endothelial cells during inflammation. The importance of ICAM-2/CD102 interactions in immune response and inflammatory diseases has driven research into their structure and function. Recombinant proteins of ICAM-2 are valuable tools for studying these interactions in detail. These proteins can be produced in cell culture systems and utilized to investigate the molecular mechanisms underlying cell adhesion, signal transduction, and immune cell trafficking. Additionally, by generating antibodies or inhibitors targeting the ICAM-2/CD102 pathway, researchers aim to explore therapeutic avenues for conditions such as asthma, atherosclerosis, and cancer, where aberrant leukocyte adhesion and migration contribute to disease pathology. Understanding the structure-function relationship of ICAM-2/CD102 through recombinant protein studies not only enhances our comprehension of immune regulation but also paves the way for developing novel immunotherapies and anti-inflammatory strategies. Overall, the study of ICAM-2/CD102 recombinant proteins is pivotal for elucidating the interactions that govern immune responses and for identifying potential clinical applications that could improve patient outcomes in various inflammatory and immunological disorders.
