Analytical Data
-
Gene name
OX40 Ligand/TNFSF4
-
简介
OX40 Ligand/TNFSF4 Protein, a cytokine, binds specifically to TNFRSF4, acting as a potent T-cell co-stimulator for proliferation and cytokine production. Operating as a homotrimer, its engagement enhances immune response by activating and expanding T-cells. The interaction with TNFRSF4 contributes to intricate regulation, crucial for modulating T-cell functions and achieving effective and controlled immune activation. OX40 Ligand/TNFSF4 Protein, Human (HEK293, mFc) is the recombinant human-derived OX40 Ligand/TNFSF4 protein, expressed by HEK293, with C-mFc labeled tag.
- Application
-
Alternative Names
OX40L; OX-40L; OX4OL; CD252; TNFSF4; OX40 Ligand; CD134 ligand; CD134L; TXGP1; Glycoprotein Gp34
-
Species
Human
-
Source
HEK293
-
Tag
C-mFc
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P23510-1
-
Expression Region
Q51-L183
-
Protein Length
Extracellular Domain
-
Molecular Weight
53-60 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
OX40 Ligand (OX40L), also known as TNFSF4, is a member of the tumor necrosis factor superfamily and plays a crucial role in regulating immune responses, particularly in T cell activation and survival. OX40L is primarily expressed on antigen-presenting cells and interacts with its receptor OX40 on T cells, leading to enhanced T cell proliferation, survival, and memory formation. The OX40/OX40L pathway has garnered significant interest in cancer immunotherapy, as it can bolster anti-tumor T cell responses. Furthermore, dysregulation of this pathway has been linked to various autoimmune diseases and inflammatory conditions. Research on recombinant OX40L protein involves its production, characterization, and assessment of its effects on T cell function in both in vitro and in vivo models. Understanding the structure-function relationship of OX40L and its interaction with OX40 provides insight into potential therapeutic applications, including the development of OX40 agonists for enhancing T cell responses against tumors or the design of antagonists to mitigate excessive immune activation in autoimmune diseases. Overall, the study of recombinant OX40 Ligand/TNFSF4 is vital for unveiling new strategies in immunotherapy and advancing the field of immune modulation.
